|Host Laboratory||FUJII LAB.|
|Position in LIMMS||PhD Student|
|Main Research Topic in LIMMS||
Bio-MEMS - Directed Evolution of Enzymes using Artificial Networks as autonomous selectors
|Keywords||DNA, Enzyme, Evolution, Droplets, Artificial networks, Microfluidic|
|Contact||LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
|adeledm at iis.u-tokyo.ac.jp|
|Short resume :|
Research Projects in Limms
1- Directed Evolution of Enzymes using Artificial Networks as autonomous selectors
- Context :
- Directed evolution is a well-established strategy that mimics the process of natural selection to discover proteins or nucleic acids with new or improved functions. After generation of a library of mutant genes, selection or screening tests are used to find the best individuals, which are then used to produce new generations of mutant. Repeating these different steps in cycles, one evolves the genetic population towards a user-defined goal.
- This technique is widely used to engineer new enzymes but presents some problematic points like the necessity of running high number of parallel tests and linking each gene to its protein activity.
- Objectives & Methods :
- The goal of the project is to design a new method based on self-replication in emulsions  and applicable to a wide range of enzymatic activities.Bacterial libraries are encapsulated with molecular programs  in monodisperse water-in-oil droplets produced thanks to microfluidics. This physically links the gene present in each bacterium to the activity of the expressed protein, which is assessed by the molecular program and converted in a genetic amplification yield. This autonomous approach does not require screening and should permit massive parallel selection tests.
Fig. 1 In vivo implementation of our approach.
Fig. 2 Fluorescent bacteria encapsulated in droplets.
- Results :
- Microfluidic device to produce 10um size droplets have been produced and used to encapsulate bacteria. Link between the protein activity and the gene amplification has been reached thanks to a simple molecular networks.
- References :
-  F. J. Ghadessy and al., Proc. Natl. Acad. Sci. U. S. A., vol. 98, no. 8, pp. 4552–4557, Apr. 2001.
-  K. Montagne, and al., Mol. Syst. Biol., vol. 7, p. 466, Feb. 2011.
Main publication List (papers, conferences and patent)
2013 and prior