Bertrand-David SEGARD, Dr.
|Host Laboratory||FUJITA LAB.|
|Position in LIMMS||Postdotoral Researcher|
Main Research Topic in LIMMS
Bio-MEMS - Impact of Pathogenic Elements of Cytoskeleton and Chemical Components on Muscle Mechanical Properties
|Nano-Tweezers, Myopathies, Bio-Mechanics|
|Contact||LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
|bdsegard at iis.u-tokyo.ac.jp|
|Short resume :|
Research Projects in Limms
1- Impact of Pathogenic Elements of Cytoskeleton and Chemical Components on Muscle Mechanical Properties
- Context :
- Myofibrillar Myopathies (MFM) are rare degenerative pathologies due to mutations within several genes. The disruption of tissues follows a slow degradation of muscular cell functions. The events leading to muscle disruption are largely unknown at biological, chemical and mechanical levels. Alteration of the mechanical characteristics of cells could be at the origin of this tissue disruption. Individual muscle cell characterization in microfluidic device offers stimulating perspectives for the study of myopathies and other cellular disorders. These differentiated cells can be studied with silicon nano-tweezers for local mechanical characteristics acquisition.
- Objectives & Methods :
- This study aims to investigate two aspects of the pathology: cell differentiation and mechanical impairment. C2C12 cells are transfected in order to express different pathogenic variants of human desmin. Then, differentiation is induced in standard conditions. Differentiated cells are isolated from the tissue and studied individually in a microfluidic device with nano-tweezers for mechanical characterization. The design of the device allows chemical stimuli. This will yield to investigate the effect of potential therapeutic compounds . Both devices used in this study have demonstrated their ability to produce results of very high quality in comparable cellular context . The combination of these technologies is an innovative way to study muscular disorders and to link genetic mutations to their consequences.
- Results :
- Previous works have described nano-tweezers and microfluidic device specific designs allowing nano-newton data acquisition. This permits experiments on isolated cell. Cells expressing different pathogenic variants of desmin (in comparable quantities) are studied under bright-field microscopy and human desmin presence is confirmed afterwards by immunofluorescence.
Fig. 1 Schematics of the silicon nanotweezers highlighting the functional structures (left panel); experimental setup showing the SNT, nanopositioning system, stimulating electrodes, culture medium in petri dish and myotubes (right panel).
Fig. 2 C2C12 cells after differentiation and before isolation. Immunofluorescence imaging of nucleus (DAPI, blue; Desmin, green).
- References :
-  Segard BD, et al., PLoS One, 2013.
-  H. Guillou, et al., APCOT 2014
Main publication List (papers, conferences and patent)
- A. Tixier-Mita, S. Ihida, B-D. Segard, G.A. Cathcart, T. Takahashi, H. Fujita, and H. Toshiyoshi, "Review on Thin-Film Transistor Technology, Its Applications, And Possible New Applications to Biological Cells," Japanese Journal of Applied Physics, March 24, 2016.
- T. Levi, A. Tixier-Mita, B-D. Segard, H. Toshiyoshi, H. Fujita, and T. Fujii, “Biomimetic Microfluidic Neurons for Hybrid Experiments,” The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, October 25-29, 2015 (poster).
- F. Delort, B-D. Segard, P. Joanne, O. Agbulut, P. Vicart, and S. Batonnet-Pichon, “Impact of Environmental Stress and New Models for Pathophysiological and Therapeutic Studies of Desminopathies,” The 20th International Congress of the World Muscle Society, WMS 2015, Brighton, United Kingdom, September 30–October 4, 2015 (poster).
- A. Tixier-Mita, S. Ihida, B-D. Segard, G.A. Cathcart, T. Takahashi, H. Fujita, and H. Toshiyoshi, “Electronic Device from TFT Display for Applications on Biological Cells,” The 47th International Conference on Solid State Devices and Materials, SSDM 2015, Sapporo, Japan, September 27-30, 2015 (presentation).
- A. Tixier-Mita, B-D. Segard, Y-J. Kim, Y. Matsunaga, H. Fujita, and H. Toshiyoshi, “TFT Display Panel Technology as a Base for Biological Cells Electrical Manipulation - Application to Dielectrophoresis,” The 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015, Estoril, Portugal, January 18-22, 2015 (poster).
2013 and prior
- B-D. Segard, F. Delort, V. Bailleux, S. Simon, E. Leccia, B. Gausseres, F. Briki, P. Vicart, and S. Batonnet-Pichon, "N-Acetyl-L-Cysteine Prevents Stress-Induced Desmin Aggregation in Cellular Models of Desminopathy," PLOS ONE, October 1, 2013.
- S. Batonnet-Pichon, B-D. Segard, F. Delort, S. Simon, E. Leccia, V. Bailleux, F. Briki, and P. Vicart, “Desmin and αB-Crystallin Mutations in Myofibrillar Myopathies: from Cellular Model to Mechanical Stress,” The 7th European Conference on Intermediate Filaments in Health and Disease, ESIFB 2011, Mykonos, Greece, June 16-19, 2011 (poster).
- S. Batonnet-Pichon, O. Agbulut, F. Bruston, O. Chourbagi, F. Delort, B-D. Segard, A. Lilienbaum, and P. Vicart, “Desmin Mutation in Myofibrillar Myopathies: Involvement of Different Physiopathological Mechanisms,” Gordon Research Conferences, Intermediate Filaments, GRC 2010, Tilton, USA, June 20-25, 2010 (poster).