Christophe PROVIN, Dr.

 C.PROVIN.jpg Host Laboratory FUJII LAB.
Position in LIMMS Postdoc JSPS (from 2004/11 to 2006/11)
Main Research Topic in LIMMS Bio-MEMS - Microfluidic experimental model of the skin for percutaneous penetration assays of cosmetics
Keywords Diffusion, On-line monitoring, Microfluidics
Contact LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6213 / Fax:+81 (0)3 5452 6213
E-mail cprovin at iis.u-tokyo.ac.jp
Download icon_pdf.gifAbstract2014_CProvin.pdf

Resume

Short resume :
2011-now Project Researcher (coll. Nihon L’Oréal): Microfluidic experimental model of the skin  |  Laboratory for Integrated Micro Mechatronics, Univ. of Tokyo
2006-2011 Project Researcher: Microanalyzer for the real-time detection of Mn2+ at the nanomolar level in deep-sea  |  Applied Microfluidics Systems Lab., Univ. of Tokyo
2004-2006 Post-Doctoral Fellow (JSPS): Development of a 3D scaffold for liver tissue engineering under perfusion conditions  |  Laboratory for Integrated Micro Mechatronics, Univ. of Tokyo
2002-2003 Teaching and Research Temporary Assistant (ATER): Application of the process developed during my Ph. D. to manufacture microdevices for chemical analysis  |  Laboratoire de Chimie-Physique et de Microbiologie pour l'Environnement / Département de Chimie-Physique des Réactions, Nancy
1999-2002 Ph. D. thesis: Development of a 3D microfabrication process based on photopolymerization to manufacture objects in polymer and ceramic materials (microstereolithography)  |  Département de Chimie-Physique des Réactions, INPL (ENSIC), Nancy
1998-1999 M.Sc. internship: Water depollution by a non-solvent process (micellar extraction)  |  Laboratoire de Chimie-Physique Organique et Colloïdale, Univ. H. Poincaré, Nancy
1998 Internship (4 months): Formulation of cutting and quenching fluids for metal processing  |  Laboratoire Metal Processing Oil, Mobil Oil Française, N.-D. de Gravenchon
Main publication List (before LIMMS)
  • Provin C., Monneret S., Le Gall H., Corbel S. Three-dimensional ceramic microcomponents made using microstereolithography. Advanced Materials, 2003, 15, 12, 994-997.
  • Monneret S., Provin C., Le Gall H., Corbel S. Microfabrication of freeform and articulated alumina-based components. Microsystem Technologies, 2002, 8, 368-374.
  • Monneret S., Provin C., Le Gall H., Corbel S. Microfabrication of freeform and articulated alumina-based components. Microsystem * Provin C., Monneret S. Complex ceramic-polymer composite microparts made by microstereolithography, IEEE Transactions On Electronics Packaging Manufacturing Part C: Manufacturing, 2002, 25, 1, 59-63.
  • Hebrant M., Provin C., Brunette J.P., Tondre C. Micellar extraction of Europium (III) by a bolaform extractant and parent compounds derived from 5-pyrazolone. Colloid and Surfaces A, 2001, 181, 225-236.

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Research Projects in Limms

1- Microfluidic experimental model of the skin for percutaneous penetration assays of cosmetics
 
Context :
With the inability to conduct cosmetic tests on animals, and the scarce availability and great variability of human skin biopsies, there is a strong need for cosmetics companies to get fast, reproducible, and low cost tests to evaluate the diffusion of new molecules through the human skin.
Objectives & Methods :
The goal is to provide a microfluidic tool to model the skin and perform those diffusion tests. The idea is to mimic the three main layers of the skin (the outermost layer, the stratum corneum; the middle layer, the epidermis; the innermost layer, the dermis) and combine it with various systems of on-line monitoring of the diffusing species.
Results :
We modelized the outermost layer of the skin as a microfluidic device (Fig. 1). The diffusion behavior and permeation coefficients obtained for different molecules (caffeine in Fig. 2, etc) are close to those reported in the literature with human skin biopsies. The inter-device reproducibility is also good. The transfer of this technology to the R&D department of L’Oréal is currently on going (patent pending).

Fig1.jpg

 Fig. 1 Microfluidic device used for percutaneous penetration assays.

Fig2.jpg

Fig. 2 Cumulative amount of caffeine diffusing through our microfluidic model of the skin.

 

2- Cell culture/ Tissue Engineering in 3D microbioreactors


   This project is centered on the use of microfabrication techniques for biological applications. Depending on the type of cells which one wishes to cultivate, the needs for those to ensure their development are different. In particular, some (as the cells of the liver) require a significant and regular contribution in nutrients and oxygen in order to keep the specific functionalities they exhibit in vivo. For liver, this contribution is being ensured in vivo by a very developed vascular network, so it can be possible to imitate that in vitro by the use of a dense network of microchannels. Moreover, this type of cells is often three dimensionally organized, which implies a structuration of the surface on which culture is done.
   Thus, the realization of an effective microbioreactor for liver cells requires the optimization of several parameters: the structure of the microbioreactor must allow a homogeneous distribution of the nutriments and oxygen together with offering surface/volume sufficient to obtain a high density of cells, and the material must be compatible with the chosen process of microfabrication plus the cell culture (biocompatibility).
   Different types of optimized structures will be tested. Those are conceived in collaboration with Shirakashi Laboratory (IIS, Univ. Of Tokyo). The microfabrication will be done with two techniques: softlithography for generation of an extruded two-dimensional PDMS (polydimethylsiloxane) reactor for ex vivo applications such as drug testing, and direct manufacture of the three-dimensional thick and complex biodegradable structures built by microstereolithography for in vivo applications such as implantable artificial liver.
The culture of HepG2 cells (human cancerous liver cells) will make it possible to determine if a high density of viable cells can be reached with those optimized microbioreactors.

Projectillustrations1.jpg

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Main publication List (papers, conferences and patent)

 2015

Journals
  1. C. Provin, A. Nicolas, S. Grégoire, T. Fujii, "A Microfluidic Diffusion Cell for Fast and Easy Percutaneous Absorption Assays", Pharmaceutical Research, Accepted (2015)
Conferences
  1. Perry, G., Xiao, W., Provin, C., Shinohara, M., Fujii, T. and Sakai, Y. Liver cord reconstruction in microfluidic device for drug screening by bile recovery : 1st steps, Symposium on New Technology for Cell-Based Drug Assay, Tokyo, Japan, January 13 2015. Poster

 

 2014-2010

Journals
  1. Provin C., Fukuba T., Okamura K., Fujii T. An integrated microfluidic system for manganese anomaly detection based on chemiluminescence: description and practical use to discover hydrothermal plumes near the Okinawa Trough. IEEE Journal of Ocean Engineering, 2013, 38, 1, 178-185.
  2. Provin C., Fujii T. Reactiondiffusion phenomena in a PDMS matrix can modify its topography. Lab on a Chip, 2011, 11, 17, 2948-2954.
Conferences

 

 

 2009 and prior

Journals
  1. Provin C., Takano K., Yoshida T., Sakai Y., Fujii T., Shirakashi R. Low O2 metabolism of HepG2 cells cultured at high density in a 3D microstructured scaffold. Biomedical Microdevices, 2009, 11, 2, 485-494.
  2. Shirakashi R., Takano K., Provin C., Sakai Y., Fujii T. Measurement of flow characteristics and oxygen metabolism of plate type bioreactor for in vitro high-density cell culture. Trans. Jpn. Soc. Mech. Eng. Ser. B, 2009, 75, 752, 864-870 (in Japanese)
  3. Fukuba T., Provin C., Okamura K., Fujii T. Development and Evaluation of Microfluidic Device for Mn Ion Quantification in Ocean Environments. IEEJ Transactions on Sensors and Micromachines, 2009, 129, 3, 69-72.
  4. Provin C., Takano K., Sakai Y., Fujii T., Shirakashi R. A method to design 3D scaffolds for high-density cell attachment and determine their optimum perfusion culture conditions. Journal of Biomechanics, 2008, 41, 7, 1436-1449.
    In « ScienceDirect’s TOP25 Hottest Articles » published in Journal of Biomechanics (april-june 2008)
  5. Yoshida T., Shirakashi R..Takano K., Provin C., Sakai Y., Fujii T. Steady Measurement of Glucose and Oxygen Consumption Rate and Cell Adhesion Ratio of HepG2 Hepatoma Cells for high Density Culture. Trans. Jpn. Soc. Mech. Eng. Ser. B, 2008, 74, 747, 2380-2386. (in japanese)
  6. Takano K., Provin C., Sakai Y., Fujii T., Shirakashi R., Fundamental Study on Mass Transport in Scaffolds for Engineering Tissues, Proc. 43rd National Heat Transfer Symposium of Japan, may 31-june 2 2006, Nagoya, Japon, Vol. II, pp. 517-518 [oral communication].
  7. Shirakashi R., Yoshida T., Provin C., Takano K., Sakai Y., Fujii T., Measurement of the energy metabolism of HepG2 cells for designing engineering tissues allowing high density cultivation, 5th World Congress of Biomechanics, July 29-August 4, Munich, Germany, 2006 [poster]
  8. Provin C.*, Takano K., Shirakashi R., Sakai Y., Fujii T., Fabrication of Microbioreactors with an Optimized Structure Designed for High Culture Density of Hepatocytes, International Conference on Microtechnologies in Medicine and Biology, Okinawa, Japan, may 9-12 2006, pp.267-270 [poster]
  9. Provin C., Monneret S., Le Gall H., Corbel S. Three-dimensional ceramic microcomponents made using microstereolithography. Advanced Materials, 2003, 15, 12, 994-997.
  10. Monneret S., Provin C., Le Gall H., Corbel S. Microfabrication of freeform and articulated alumina-based components. Microsystem Technologies, 2002, 8, 368-374.
  11. Provin C., Monneret S. Complex ceramic-polymer composite microparts made by microstereolithography, IEEE Transactions On Electronics Packaging Manufacturing Part C: Manufacturing, 2002, 25, 1, 59-63.
  12. Provin C., Monneret S., Le Gall H., Corbel S. Mise en forme de micro-objets composites polymère/céramique créés par microstéréolithographie. Entropie, 2001, 235/236, 90-95. (in french)
  13. Hébrant M., Provin C., Brunette J.P., Tondre C. Micellar extraction of Europium (III) by a bolaform extractant and parent compounds derived from 5-pyrazolone. Colloid and Surfaces A, 2001, 181, 225-236.
Conferences
  1. Shirakashi, R.,Yoshida, T.,Provin, C.,Takano, K.,Sakai, Y. and Fujii, T., " Measurement of the energy metabolism of HepG2 cells for designing engineering tissues allowing high density cultivation &quot, in Proc. of The 5th World Congress of Biomechanics, 2006, Munich, Germany, 29 July - 4 August, CD

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