Elsa Mazari, Dr.
|Host Laboratory||TAKEUCHI LAB.|
|Position in LIMMS||Postdoctoral Researcher|
|Main Research Topic in LIMMS||
Bio-MEMS - Microfluidic devices for cellular tissue encapsulation
3D culture, biological materials, tissue engineering
|Contact||LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
|mazari at iis.u-tokyo.ac.jp|
|Short resume :|
|2014-2016||JSPS Postdoctoral fellowship in Shoji Takeuchi Laboratory, University of Tokyo, Tokyo.|
|2009-2014||PhD in the Photonic and Nanostructures Laboratory/Jacques Monod Institute/Ecole Normale Superieure, Paris.|
|2008-2009||MS in Photonic and Nanostructures Laboratory/Jacques Monod Institute, Paris|
Research Projects in Limms
1- Microfluidic devices for cellular tissue encapsulation
- Context :
- Microfluidic devices are well suited to accurately position cells and tissues, control cell shape and function, and so create highly structured 3D culture microenvironments. Indeed, 3D cell-culture devices promote levels of cell differentiation and tissue organization not possible in conventional 2D culture systems. Unfortunately, most studies on cell and tissue regulation have relied on analysis of cells grown in 2D cell-culture models that partially restrict cell spreading and that fail to reconstitute the in vivo cellular microenvironment.
- Objectives & Methods:
- We here propose a double-coaxial laminar flow microfluidic device which produces tuned 3D cell-laden structures of various shapes with different mechanical and biochemical properties that may be used as therapeutic vectors notably for the cell transplantation .
- Results :
- We have produced various 3D microfibers (cf. Fig.1) that encapsulate cells and ECM proteins optimally replicating intrinsic functionalities of tissues with a minimal amount of specific biological materials . We aim to highlight those preliminary and promising achievements by optimizing biological and chemical materials used for microfibers and also by implementing innovating fabrication techniques for microfluidic device. These improvements will permit to achieve cell-laden microfibers that exhibit optimal tissue morphologies and functions. We finally intend to realize in vivo investigations developing both safe and dedicated protocols for cell transplantation applications in order to demonstrate the relevance of artificial cell-fibres.
- Fig. 1 Image of a NIH-3T3 cells microfiber after 3 days of culture. Scale bar, 200 µm.
- References and publications :
-  H. Onoe, et al., Nat.Mat, 12, 2013.
Main publication List (papers, conferences and patent)
- E. Mazari-Arrighi, T. Okitsu, M. Kiyosawa, H. Aoyagi, M. Yano, and S. Takeuchi. 3D culture of primary hepatic cells using cell-fiber technology for long-term maintenance of liver-specific functions in vitro. MicroTAS 2016, the 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp. 433-434.
- C. Gosse, X. Zhao, A. Perea-Gomez, I. Hue, S. Degrelle,I. Migeotte, D. Suárez-Boomgaard, and E. Mazari-Arrighi. Electroporation to Study Embryology Morphogenesis and Organogenesis. Handbook of Electroporation, Section: Laboratory Techniques Used in Electroporation Research, Springer, in press.
- X. Zhao, D. Suárez-Boomgaard, E. Mazari-Arrighi, J. Safioui, I. Migeotte, A. Perea-Gomez, and C. Gosse. Engineering a reusable glass device to electroporate a few cells and study their migration in the developing mouse embryo. MicroTAS 2015, the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp. 671-673.
- E. Mazari-Arrighi, T. Okitsu, M. Kiyosawa, H. Aoyagi, M. Yano, and S. Takeuchi. Controlled fabrication of fibre-shaped microstructures to promote high-density three dimensional culture of rat primary hepatocytes. MicroTAS 2015, the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp. 579-581.
2013 and prior