Filiz YESILKOY, Dr.

FilizYesilkoy.jpg Host Laboratory KIM LAB.
Position in LIMMS EUJO-LIMMS Researcher (EPFL)
Main Research Topic in LIMMS

Bio-MEMS - Microfluidic Cell Trapping Methods for Single Cell Analysis for Lab-on-a-chip Applications

Keywords

Microfluidics, Biosensing, Heat-Shock-Proteins, Micro-fabrication

Contact

LIMMS/CNRS-IIS (UMI 2820) 
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan 
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088

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EPFL Ecole Polytechnique Federale de Lausanne
Institute of Microengineering, EPFL STI IMT LMIS-1, BM 3202 Station 17
CH - 1015 Lausanne, Switzerland
Direct : +41-21-693.65.73 / Sec : +41-21-693.59.01 (S. Eggli)

E-mail filiz at iis.u-tokyo.ac.jp
Download icon_pdf.gifAbstract2014_FYesilkoy.pdf, Abstract2016

Resume

Short resume :
2012-now Actual position
2008-2012 Here
2004-2008 Here
2001-2003 Here

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Research Projects in Limms

1- Microfluidic Cell Trapping Methods for Single Cell Analysis for Lab-on-a-chip Applications

Context :
Manipulation of the individual cells on a microfluidic device has revolutionized the traditional population based cell culture experiments removing the negative aspects of statistical data averaging [1]. Moreover, simultaneous observation of the cells on addressable array locations opened the paths for the investigation of unknown cell expressions and intercellular communication mechanisms. For example, using the proposed devices single-cell analysis of Heat Shock Response (HSR) in eukaryotic cells (particular protein expression of cells to prevent the damage triggered by the stressors) can lead to the crucial development of HSR inhibitors in cancer research.
Objectives & Methods :
This work includes the investigation of microfluidic cell trapping methods optimized for various cell-types and experiments. Single-cell isolating microfluidic devices are designed, fabricated using standard micro-fabrication methods and tested using live cells. As a biological application, Heat Shock Response (HSR) of a genetically modified cell line (NIH/3T3) is used to first immobilize cells individually on the micro-structured cell trap stations located in a microfluidic channel where the cells are cultured and examined.
Results :
Cell isolation on the hydrodynamic array trapping devices is shown successfully using the fluorescent dyed U-937 lymphomas. Fig. 1 shows an optical image of the hydrodynamic cell trap array. With this method 95 % trapping efficiency is achieved on average. The heat shock response on the individually isolated NIH/3T3 cells using micro-well trapping method is also successfully demonstrated.  Fig. 2 shows the superposition of fluorescent and optical images of a sample array on the fifth hour after heat stressing the cells.

Fig1_copy.jpg

Fig. 1 Optical image of the arrayed hydrodynamic cell trap stations.

More than 95% trapping efficiency is achieved repeatedly on an array of 500 cell trap stations.

Fig_2_copy.jpg

Fig. 2 HSR expression of the micro-well trapped NIH/3T3 cells.

References :
[1] S. H. Kim, et al., Single-Cell Analysis, Humana Press, 2012
[2]P. Ginet, et al., Lab. Chip, vol. 11, no. 8, pp. 1513–1520, 2011.

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Main publication List (papers, conferences and patent) 

2013

Journals
Conferences

 

2012

Journals
Conferences

 

2011

Journals
Conferences

 

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