Florian LARRAMENDY, Dr.

 F.Larramendy.jpg Host Laboratory TAKEUCHI LAB.
Position in LIMMS EUJO-LIMMS Researcher (IMTEK)
Main Topic Research in LIMMS

Bio-MEMS - Polymer-based microstructures for controlled 2D and 3D cell networks

Keywords

Polymer structures, Cell network, Immobilization, Biosensors

Contact

LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088

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IMTEK - Department of Microsystems Engineering
Microsystem Materials Laboratory (MML), University of Freiburg
Georges-Koehler-Allee 103, 79110 Freiburg, Germany

E-mail flarrame at iis.u-tokyo.ac.jp
Download icon_pdf.gifAbstract2015_FLarramendy.pdf , Abstract2016

Resume

Short resume :
2012-now Actual position
2008-2012 Here
2004-2008 Here
2001-2003 Here

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Research Projects in Limms

1- Polymer-based microstructures for controlled 2D and 3D cell networks
 
Context :
The analysis of neuronal cells embedded in networks of controlled geometry has grown rapidly over the past decade and stays important for biological studies and medical research. The positioning of individual cells has become a key technique for cell engineering applications such as cell therapy and brain regeneration.
Objectives & Methods :
We developed different kinds of polymer-based microstructures for positioning and fixing cells in specific places. Cells could develop extensions freely or with guidance, and create a network in two or three dimensions. Several approaches, based on various structures and polymers, have been investigated: mechanical constraint, high-topography surface functionalization and stackable structures. We are currently working on the last approach for controlled 3D cell network [1]. The long-term objective is to create a hybrid system (electronic and polymer-based structures) for biological and medical applications.
Results :
We successfully developed an innovative technique, previously used in microfluidics, to realize honeycomb arrangements of cell containers interconnected by microchannels using SU-8. These structures are fabricated using a single UV-photolithography exposure. Each container is centered on a nanopillar array for cell positioning [2], as shown Figure 1. We have also developed a new technique for patterning functionalization layers on substrates with high topography [3]. The technique is based on the peel-off of a parylene layer deposited on a structured sacrificial photoresist. We have successfully demonstrated the guided growth of neuron-like PC12 cells on different patterns of cell-growth promoting proteins on micro­pillars, in microwells, and in-between, as shown in Figure 2.

Fig.1_Larramendy.png

Fig. 1 Scanning electron micrograph of a neuron on a nanopillar array with neurite growth.

Fig.2_Larramendy.png

Fig. 2 (a) Optical micrograph of neuron-like PC-12 cells grown on micropillar arrays with neurites guided by laminin lines to form ‘MEMS’ and (b) its equivalent in fluorescence microscopy after live-dead test.

References :
[1] F. Larramendy et al., IEEE Transducers 2015.
[2] F. Larramendy et al., J. Micromech. Microeng, 2015.
[3] F. Larramendy et al., IEEE MEMS 2015.

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Main publication List (papers, conferences and patent)

2016

Journals
  1. F. Larramendy, S. Yoshida, L. Jalabert, S. Takeuchi and O. Paul, Surface Modification for Patterned Cell Growth on Substrates with Pronounced Topographies using Sacrificial Photoresist and Parylene-C Peel-off, Journal of Micromechanics and Microengineering, Volume 26, 2016, 095017 (10pp)
Conferences

 

2015

Journals
  1. F. Larramendy et al., J. Micromech. Microeng, 2015.
Conferences
  1. F. Larramendy, D. Serien, S. Yoshida, L. Jalabert, S. Takeuchi and O. Paul, "High-topography surface functionalization based on parylene-C peel-off for patterned cell growth", The 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2015), January 18-22, 2015, Estoril (Portugal)

 

2014

Journals
  1. F. Larramendy et al., Biosensors 2014
Conferences

 

 

2013 and prior

Journals
  1. F. Larramendy et al., Lab on a chip 12, 387, 2012
Conferences

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