Host Laboratory	FUJITA LAB.
	Position in LIMMS	PhD Student
	Main Research Topic in LIMMS	Bio-MEMS - Silicon Nanotweezers for the characterization of DNA damage under therapeutic radiation beams
	Keywords	Nanotweezers, DNA damage characterization, Radiotherapy
Contact	LIMMS/CNRS-IIS (UMI 2820) Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
E-mail	gperret at iis.u-tokyo.ac.jp
Download	Abstract2015_GPerret.pdf

Resume

Short resume :
2012-now	Actual position
2008-2012	Here
2004-2008	Here
2001-2003	Here

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Research Projects in Limms

1- Silicon Nanotweezers for the characterization of DNA damage under therapeutic radiation beams

Context :

Tumor cell killing in cancer radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. It is well assessed that radiation induces single-strand and double-strand breaks in DNA, by various mechanisms [1], whose details and efficacy are, however, largely unknown.

Objectives & Methods :

Study the mechanics of DNA damage under ionizing beams for optimized tumor treatment. The Silicon Nano Tweezers (SNT) is a MEMS device for direct manipulation of biomolecules [2], an excellent candidate for in-beam operation thanks to its tiny size. The SNT, Fig.1.a, comprise two parallel arms ending with sharp tips, designed to trap molecules. SNT + DNA in inserted inside microfluidic cavity (Fig.1.b) and placed under radiotherapy machine (Cyberknife). The mechanical characteristic of the trapped molecules (stiffness, viscosity) under X-ray are measured in real time by monitoring the resonance frequency of the SNT handling the molecules, Fig.2.

Results :

Silicon Nanotweezers operation under therapeutic irradiation conditions, and direct detection of DNA damage inside DI water under X-ray beam was demonstrated for the first time.

Fig.1 a) SNT + DNA Bundle b) SNT Oscillator model c) Cyberknife

Fig.2 Mechanical characterization of DNA bundle damage during irradiation

References :

[1] R. E. Krisch, M. B. Flick, and C. N. Trumbore, Radiation Research, vol. 126, no. 2, pp. 251–259, 2013.

[2] G. Hashiguchi, T. Goda, M. Hosogi, K. Hirano, N. Kaji, Y. Baba, K. Kakushima, and H. Fujita, Anal. Chem., vol. 75, no. 17, pp. 4347–4350, 2003.

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Main publication List (papers, conferences and patent)

2015

Journals

Conferences

 

 

2014

Journals

Conferences

1. F. Manca, S. Giordano, P.L. Palla, G. Perret, E. Lartigau, D. Collard, H. Fujita, F. Cleri, "Theoretical study of the basic mechanisms of DNA damage by therapeutic radiation beams", E-MRS Spring meeting 2014, Symposium N, May 26-29, Lille (France).
2. G. Perret, T. Lacornerie, M. Kumemura, H. Guillou, L. Jalabert, E. Lartigau, T. Fujii, F. Cleri, H. Fujita, D. Collard, "Silicon nanotweezers inside liquid for the real time characterization of DNA degradation under radiotherapy treatment", E-MRS Spring meeting 2014, Symposium N, May 26-29, Lille (France).
3. P-T. Chiang, M. Kumemura , H.Fujita, G. Perret, N. Lafitte, L.Jalabert, D. Collard, A. Enomoto, K. Miyagawa, Real-time Measurement of DNA Degradation under Radiation by Silicon Nanotweezers Coupled with Microfluidic Cavity, Technical Meeting on Sensors and Micromachines 2014.

 

2013 and prior

Journals

Conferences

1. G. Perret, T. Lacornerie, M. Kumemura, N. Lafitte, H. Guillou, L. Jalabert, E. Lartigau, T. Fuji, F. Cleri, H. Fujita, D. Collard, Real Time Biomechanical Characterization of DNA Damage under Therapeutic Radiation Beams, MRS Boston, Dec. 3rd 2013 (Oral).
2. G.Perret, T.Lacornerie, M.Kumemura, N.Lafitte, H.Guillou, L.Jalabert, E.Lartigau, T.Fujii, F.Cleri, H. Fujita, D.Collard , Real time bio mechanical characterization of DNA damage under therapeutic radiation beams,  MicroTAS 2013, October 27-31, 2013, Freiburg, Germany. (poster)
3. G. Perret, Silicon Nanotweezers with a Microfluidic Cavity for the Real Time Characterization of DNA Damage under Therapeutic Radiation Beams, EMBC'13, July 3-7, 2013, Osaka, Japan.(Oral)

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