Guillaume GINES, Dr.
|Host Laboratory||FUJII LAB.|
|Position in LIMMS||Postdoctoral Researcher|
|Main Research Topic in LIMMS||
Bio-MEMS - DNA-Programmable particles: toward in vitro models of biological collective behaviors
DNA-functionalized microparticles, Biological mimics, Molecular programming, Microscopy
|Contact||LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
|ginesg at iis.u-tokyo.ac.jp|
|Short resume :|
Research Projects in Limms
1- DNA-Programmable particles: toward in vitro models of biological collective behaviors
- Context :
- In living systems, the computations performed at the molecular level drive the macroscopic behavior of cells and organisms. Similarly, information exchange between individuals or cells in a group eventually triggers collective behaviors (e.g. within brains, organisms or ecosystems). We aim at reproducing and exploring these phenomena using DNA-programmable particles as cell or organism models.
- Objectives & Methods :
- We adapted the solution-phase Polymerase/Exonuclease/Nickase Dynamic Networks Assembly toolbox (PEN DNA)1,2 toolbox to program the behavior of microscopic particles, themselves embedded in a population of cross-interacting agents (Fig. 1). To that purpose, DNA-encoded programs (templates) are tethered to microbeads conferring them the ability to receive and send information (as short DNA single strands) but also locally compute according to their own network dynamic.
- Results :
- In-solution DNA programs were successfully transposed on particles and run with supported templates, given minor adjustments. Programmed beads exhibit an autonomous reactivity and display various features such as monostability, bistability or synergy. Networks of particles reveal spatially-resolved collective behaviors such as travelling front propagation (Fig. 2). The dynamics in such a system is similar to an infectious spread in epidemiological models.
Fig. 1 Mesoporous micropaticles are programmed with DNA templates (A) in order to build networks and create collective behaviors (B).
Fig. 2 Traveling front propagation across a population of bistable microparticles.
- References :
- Montagne, K. et al. Programming an in vitro DNA oscillator using a molecular networking strategy. Mol. Syst. Biol. 7, 466 (2011).
- Baccouche, A. et al. Dynamic DNA-toolbox reaction circuits: A walkthrough. Methods 67, 234–249 (2014).
Main publication List (papers, conferences and patent)
2013 and prior