Herve GUILLOU, Dr.
|Host Laboratory||FUJII LAB.|
|Position in LIMMS||CNRS Researcher (from 2010)|
|Main Research Topic in LIMMS||
Bio-MEMS - Bio-mechanical diagnostics at the cell level using silicon nano tweezers
|Keywords||Nanotweezers, Cell, Bio-mechanics|
|Contact||LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
|mailto: guillou at iis.u-tokyo.ac.jp|
|Short resume :|
Research Projects in Limms
1-Bio-mechanical diagnostics at the cell level using silicon nano tweezers
- Context :
- Cell mechanical properties are altered in numerous pathologies and a quantitative and specific characterization gives useful clues on the origin or stage of the pathology. In the field of tissue engineering, mechanical characterization of the tissue is required to evaluate the functionality of the tissues. Our projects aims at providing with MEMS the versatile, simple and sensitive tools to perform biomechanical diagnostic at the single cell level.
- Objectives & Methods:
- The objectives are to optimize the nanotweezers in order to improve their sensitivity down to the sub-nanonewton range, to integrate smart microfluidics that allow stable operation, to add new functionalities required for the biomechanical testing of single cells and to provide an automated and integrated experimental platform for fast and reliable operation.
- Results :
- The designed experimental platform with integrated robotics fits onto an inverted and fluorescence microscope (fig1). We can reproducibly approach spread cells (height few 100 nm) and cell in suspension to perform biomechanical characterization . Nanotweezers positioning at the subcellular scale is achieved (fig2). The nanotweezers have been instrumented with two active arms and a lower stiffness in order to improve the force resolution . The stroke of the nanotweezers reaches now 20 µm with a resolution of the ordre of 1 nm making them suitable for the characterization of ultra soft materials.
Fig. 1 Schematics of the setup used to perform simultaneous mechanical characterization on myotubes and electrical stimulation.
Fig. 2 Experiment done on living spread cell, the cell cortex is indented at several places showing irreversible transformations suggesting that the cell cytoskeleton has flown.
- References :
-  H. Guillou et al. NMC conference, Stanford (2013)
-  H. Guillou et al. Transducer 2013, Barcelonna (2013)
Main publication List (papers, conferences and patent)
1. H. Guillou et al. NMC conference, Stanford (2013)
2. H. Guillou et al. Transducer 2013, Barcelonna (2013)