Mathieu DANOY

 mathiu_danoy.jpg Host Laboratory SAKAI LAB.
Position in LIMMS EUJO-LIMMS PhD Student
Main Research Topic in LIMMS

Bio-MEMS - Development of an in-vitro model for marker-free, real time monitoring of the cancer cell invasion into tissues

Keywords

Cancer, Metastasis, Cell-based assay, Impedance spectroscopy

Contact LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
E-mail  mdanoy at iis.u-tokyo.ac.jp
Download icon_pdf.gifAbstract2015_MDanoy.pdf , Abstract2016

Resume 

Short resume :
2014-Present PhD degree
LIMMS, Institute of Industrial Science, The University of Tokyo
University of Lille 1, Engineering Science department
Hosted in Sakai Yasuyuki lab., Institute of Industrial Science, The University of Tokyo.
Thesis title: Development of an in-vitro model for real-time, marker-free monitoring of the invasion of cancer cells into tissues.
2013-2014 Master degree, specialty Micro and NanoTechnologies(MNT)
University of Lille 1
2009-2014 Engineering diploma, Specialty: Digital Technology and Applications
ISEN Lille, electronics and computer science engineering school

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Research Projects in Limms

1- Development of an in-vitro model for marker-free, real time monitoring of the cancer cell invasion into tissues

Context :
Cancer metastasis is a complex phenomenon defined by several steps. The cancer cell originated from a primary site will migrate through vessels and attain a secondary site where it will form metastasis. In this project, we focus on designing a model to study the cancer cell intravasation detailed in fig. 1, the process in which the cancer cell rolls on the walls of a blood vessel, attach to it and finally migrate through it [1]. For drug-screening purposes, a reliable, human-specific model is needed.
Objectives & Methods :
Our first objective is to build an in-vivo like liver microvasculature using coculture. To be as close as possible to the actual in-vivo situation,  we need our coculture to be as complete as possible by including not only endothelial cells and hepatocytes but also pericytes (Fig. 2). The viability of the model will be checked by measuring the expression of different endothelial markers.
Once a satisfying model has been designed, the adhesion and migration of the cancer cell through the endothelium will be studied by impedance spectroscopy and linked to fluorescence microscopy observations thus giving us a label-free, quantitive method to monitor those phenomenons.

 Fig1_Danoy.jpg

Fig. 1 Cancer cell intravasation process.

 Fig2_Danoy.jpg

Fig.2 Example of studied coculture system.

Results:
Previous studies in our laboratory [2] didn’t include the complete coculture system as we plan to do it but it was observed that the use of liver specific endothelial cells would influence the adhesion of different cancer cells accordingly to their probability of metastasizing in the liver.
Trials are now performed by varying the geometry of the full coculture in order to find an highly reproducible liver-mimicking system. The next step is the inclusion of this coculture in micro-systems.

References :
[1] Chaffer, C. L., & Weinberg, R. A. (2011). A perspective on cancer cell metastasis. Science, 331(6024), 1559-1564.
[2] Chowdhury, M. M., Danoy, M., Rahman, F., Shinohara, M., Kaneda, S., Shiba, K., ... & Sakai, Y. (2014). Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International, 2014.

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Main publication List (papers, conferences and patent)

2016

Journals
  1. Pang, Y., Horimoto, Y., Sutoko, S., Montagne, K., Shinohara, M., Danoy, M., ... & Sakai, Y. (2016). Novel integrative methodology for engineering large liver tissue equivalents based on three-dimensional scaffold fabrication and cellular aggregate assembly. Biofabrication, 8(3), 035016.

 

Conferences
  1. Mathieu Danoy, Marie Shinohara, Astia Rizki-Safitri, Dominique Collard, Vincent Senez, Yasuyuki Sakai, A novel hierarchical in-vitro coculture model of the liver microvasculature for pancreatic cancer cells adhesion monitoring, TERMIS-AP 2016, Tamsui, Taiwan, September 2016, Best poster award

 

2015

Journals

 

Conferences

 

2014

Journals
  1. Chowdhury, M. M., Danoy, M., Rahman, F., Shinohara, M., Kaneda, S., Shiba, K., ... & Sakai, Y. (2014). Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International, 2014.
Conferences

 

2013 and prior

Journals

 

Conferences

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