|Host Laboratory||SAKAI LAB.|
|Position in LIMMS||EUJO-LIMMS PhD Student|
|Main Research Topic in LIMMS||
Bio-MEMS - Development of an in-vitro model for marker-free, real time monitoring of the cancer cell invasion into tissues
Cancer, Metastasis, Cell-based assay, Impedance spectroscopy
|Contact||LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
|mdanoy at iis.u-tokyo.ac.jp|
|Download||Abstract2015_MDanoy.pdf , Abstract2016|
|Short resume :|
LIMMS, Institute of Industrial Science, The University of Tokyo
University of Lille 1, Engineering Science department
Hosted in Sakai Yasuyuki lab., Institute of Industrial Science, The University of Tokyo.
Thesis title: Development of an in-vitro model for real-time, marker-free monitoring of the invasion of cancer cells into tissues.
|2013-2014||Master degree, specialty Micro and NanoTechnologies(MNT)
University of Lille 1
|2009-2014||Engineering diploma, Specialty: Digital Technology and Applications
ISEN Lille, electronics and computer science engineering school
Research Projects in Limms
1- Development of an in-vitro model for marker-free, real time monitoring of the cancer cell invasion into tissues
- Context :
- Cancer metastasis is a complex phenomenon defined by several steps. The cancer cell originated from a primary site will migrate through vessels and attain a secondary site where it will form metastasis. In this project, we focus on designing a model to study the cancer cell intravasation detailed in fig. 1, the process in which the cancer cell rolls on the walls of a blood vessel, attach to it and finally migrate through it . For drug-screening purposes, a reliable, human-specific model is needed.
- Objectives & Methods :
- Our first objective is to build an in-vivo like liver microvasculature using coculture. To be as close as possible to the actual in-vivo situation, we need our coculture to be as complete as possible by including not only endothelial cells and hepatocytes but also pericytes (Fig. 2). The viability of the model will be checked by measuring the expression of different endothelial markers.
- Once a satisfying model has been designed, the adhesion and migration of the cancer cell through the endothelium will be studied by impedance spectroscopy and linked to fluorescence microscopy observations thus giving us a label-free, quantitive method to monitor those phenomenons.
Fig. 1 Cancer cell intravasation process.
Fig.2 Example of studied coculture system.
- Previous studies in our laboratory  didn’t include the complete coculture system as we plan to do it but it was observed that the use of liver specific endothelial cells would influence the adhesion of different cancer cells accordingly to their probability of metastasizing in the liver.
- Trials are now performed by varying the geometry of the full coculture in order to find an highly reproducible liver-mimicking system. The next step is the inclusion of this coculture in micro-systems.
- References :
-  Chaffer, C. L., & Weinberg, R. A. (2011). A perspective on cancer cell metastasis. Science, 331(6024), 1559-1564.
-  Chowdhury, M. M., Danoy, M., Rahman, F., Shinohara, M., Kaneda, S., Shiba, K., ... & Sakai, Y. (2014). Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International, 2014.
Main publication List (papers, conferences and patent)
- Pang, Y., Horimoto, Y., Sutoko, S., Montagne, K., Shinohara, M., Danoy, M., ... & Sakai, Y. (2016). Novel integrative methodology for engineering large liver tissue equivalents based on three-dimensional scaffold fabrication and cellular aggregate assembly. Biofabrication, 8(3), 035016.
- Mathieu Danoy, Marie Shinohara, Astia Rizki-Safitri, Dominique Collard, Vincent Senez, Yasuyuki Sakai, A novel hierarchical in-vitro coculture model of the liver microvasculature for pancreatic cancer cells adhesion monitoring, TERMIS-AP 2016, Tamsui, Taiwan, September 2016, Best poster award
- Chowdhury, M. M., Danoy, M., Rahman, F., Shinohara, M., Kaneda, S., Shiba, K., ... & Sakai, Y. (2014). Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo. BioMed Research International, 2014.
2013 and prior