Mehmet Cagatay TARHAN, Dr.
|Host Laboratory||FUJITA LAB.|
|Position in LIMMS||EUJO-LIMMS Researcher (UT-IIS)|
|Main Research Topic in LIMMS||
Advanced-MEMS - Integrating silicon nanotweezers with microfluidics for biomechanical characterization of macromolecules
Microfluidics, nanotweezers, macromolecules, biosensing
|Contact||LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
|mctarhan at iis.u-tokyo.ac.jp|
|Download||Abstract2015_MCTarhan.pdf , Abstract2016|
Research Projects in Limms
1- Integrating silicon nanotweezers with microfluidics for biomechanical characterization of macromolecules
- Context :
- Performing bioassays with mechanical actuators often suffer from a trade-off between actuation performance and bioassay compatibility. Optimal performance of actuators prevents them from working in liquid. However, majority of biological experiments take place in liquid. MEMS technology provides mechanical actuations with electrical readouts showing supreme merits for characterizing mechanical properties of biomolecules. Therefore, a new kind of microfluidic approach should be developed to perform in-liquid biological experiments without compromising the optimal in-air performance of MEMS actuators.
- Objectives & Methods :
- This work aims at developing a microfluidic system for silicon nano tweezers (SNT) to perform rapid routine test for mechanical properties of macromolecules such as DNA and microtubules. Proposed system provides the necessary platform to test multiple solutions on the same molecular bundle captured between SNT tips. To achieve this goal, a microfluidic device with a microchannel was fabricated. In addition to an inlet and an outlet, the channel had an additional opening at the side for SNT tips to enter inside. Due to the dimensions and the materials, the opening was stable throughout the experiments.
- Results :
- Measurements performed with bare SNTs indicated the high-stability performance of the system. Long-term measurements (>10 hours) were successfully performed without compromising the stability. Similarly, repeated exchange of the liquid inside the channel was succeeded. For demonstration purposes, the system was used to test mechanical properties of macromolecules using DNA bundles. Stiffness and viscos losses of the bundles were tested using solutions with different pH levels. Considering the fast preparation and measurement time, the results showed that the proposed system could be used as a real-time routine test to investigate the mechanical properties of biomacromolecules.
Fig. 1 Schematic view of the proposed setup integrating SNT and microfluidics.
Fig. 2 An image showing the tips of SNT entering into the microchannel.
- References :
-  M. C. Tarhan, et al., MicroTAS’12, pp. 1897, 2012.
Main publication List (papers, conferences and patent)
- S.L. Karsten, M.C. Tarhan, L.C. Kudo, D. Collard, H. Fujita, Point-of-Care Devices (POCDs) by Means of Advanced MEMS, Talanta, 2015 (accepted).
- Tarhan, M. C., Yokokawa, R., Morin, F. O. and Fujita, H. (2013), Specific Transport of Target Molecules by Motor Proteins in Microfluidic Channels. ChemPhysChem, 14: 1618–1625. doi: 10.1002/cphc.201300022.
2012 and prior
- Tarhan M.C.,Yokokawa R.,Bottier C.,Collard D. and Fujita H., A Nano-needle/microtubule composite gliding on a kinesin-coated surface for target Molecule Transport, Lab on a chip, 10(1): 86-91, 2010.
- Bottier C., Fattaccioli J.,Tarhan M.C.,Yokokawa R.,Morin F.O.,Kim B.J.,Collard D. and Fujita H., Active transport of oil droplets along oriented microtubules by kinesin molecular motors, Lab on a chip, 9(12): 1694-1700, 2009.
- F.O. Morin, F. Rose, P. Martin, M.C. Tarhan, H. Kawakatsu, H. Fujita, "Combing and Self-assembly Phenomena in Dry Films of Taxol-Stabilized Microtubules", Nanoscale Research Letters, 2007, Volume 2, pp135-143.
- Morin, F.O.,F, Rose,P, Martin,Tarhan, M.C.,Kawakatshu, H. and H, Fujita, " Combing and self-assembly phenomena in dry films of Taxol-stabilized microtubules " Nanoscale Research Letters, 2006, Accepted for publication.
- M. C. Tarhan, D. Collard, L. Jalabert, M. Kumemura, N. Lafitte, Q. Delouvee, S. L. Karsten and H. Fujita, "CONTINUOUS REAL-TIME MONITORING OF MOLECULAR DETECTION BY SILICON NANOTWEEZERS-INTEGRATED MICROFLUIDIC DEVICE", MicroTAS, Okinawa, JAPAN, Oct. 28 – Nov. 1, 2012.
- M. C. Tarhan, R. Yokokawa, L. Jalabert, D. Collard, and H. Fujita, "Assembling Microtubule Networks for a Motor Protein-based Nano Transport System", 2011 MRS Spring Meeting and Exhibit, San Francisco, USA, Apr 26, 2011
- M. C. Tarhan, R. Yokokawa, L. Jalabert, D. Collard and H. Fujita, A MOTOR PROTEIN-BASED ENZYMATIC DETECTION SYSTEM, accepted to MicroTAS 2011.
- MC. Tarhan, R. Yokokawa, L. Jalabert, D. Collard, H. Fujita, Biomotor-Based Nanotransport System Constructed by Pick-And-Place Assembly of Individual Molecule, The 2010 IEEE/RSJ International Conference on Intelligent RObots and Systems, Taipei International Convention Center, Taipei, Taiwan, October 18-22, 2010
- Bottier C.,Fattaccioli J.,Tarhan M.C.,Yokokawa R.,Kim B.,Collard D. and Fujita H., Kinesin-driven oil droplets used as carriers for transportation of solid particles, 13th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 09, Jeju, Korea, 2009.
- Tarhan M.C.,Jalabert L.,Yokokawa R.,Bottier C.,Collard D. and Fujita H., Nano monorail for molecular motors: individually manipulated microtubules for kinesin motion, 15th Int. Conf. on Solid State Sensors, Actuators and Microsystems, Transducers 09, Denver, USA, 2164-2167, 2009.
- Wada K.,Tarhan M.C.,Bottier C.,Collard D.,Fujita H. and Yokokawa R., Fabrication and characterization of multiple nanowires using microtubule structures, 15th Int. Conf. on Solid State Sensors, Actuators and Microsystems, Transducers 09, Denver, USA, 2009
- Bottier, C.; Tarhan, M. C.; Collard, D.; Yokokawa, R.; Fujita, H. Kinesin-based Transportation and Electrofusion of Lipid Vesicles, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS'08, San Diego, USA, Oct. 12-16, 2008.
- Tarhan, M. C.; Collard, D.; Bottier, C.; Yokokawa, R.; Hosogi, M.; Hashiguchi, G.; Fujita, H. Isolation and Manipulation of Single Microtubule by Silicon Microtweezers, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS'08, San Diego, USA, Oct. 12-16, 2008.
- Bottier, C.; Tarhan, M. C.; Fattaccioli, J.; Morin, F. O.; Kim, B. J.; Fujita, H. Direct Transportation and Electrofusion of Oil Droplets in a Microfluidic Device, 21st IEEE Int. Conf. on Microelectromechanical Systems, MEMS'08, Tucson, USA, Jan. 13-17, 2008.
- Tarhan, M. C.; Yokokawa, R.; Bottier, C.; Sarajlic, E.; Fujita, H. Transport of Functionalized Microneedles by Gliding Assay-based Molecular System, 4th Asia-Pacific Conference on Transducers and Micro-Nano Technology, APCOT'08, Tainan, Taiwan, Jun. 22-25, 2008.
- Yokokawa, R.; Tarhan, M. C.; Bottier, C.; Collard, D.; Fujita, H.; Kasahara, M. DNA Molecule Manipulation by Motor Proteins and Enzymatic Reaction at the Single Molecule Level, 25th Sensor Symposium on Sensors, Micromachines, and Applied Systems, Okinawa, Japan, Oct.22-24, 2008.
- Tarhan, M.C. ,Yokokawa, R.,Morin, F,Takeuchi, S,Kon, T. and Fujita, H, " Carrying target molecules on beads by bio-molecular motors ", in Proc. of MEMS'06, 2006, Istanbul, Turkey, 22-26 January, 526-529
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