Myriam Lereau Bernier, Dr.
|Host Laboratory||SAKAI LAB.|
|Position in LIMMS||Postdoctoral Researcher|
|Main Research Topic in LIMMS||
Bio-MEMS-Development of a new microscale culture model to investigate the differentiation and maturation of induced pluripotent stem (iPS) cells to hepatocytes
iPS, hepatocytes, liver-on-chip, bioreactor
|Contact||LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
|myriam at iis.u-tokyo.ac.jp|
|Short resume :|
|2016-now||JSPS postdoctoral researcher (LIMMS, Tokyo)|
|2013-2014||CNRS IR (SysDiag – Bio-Rad/CNRS, Montpellier, France)|
|2011-2013||Postdoctoral researcher (Etablissement Français du Sang, Montpellier, France)|
|2006-2010||PhD student (INSERM – International Agency for Research on Cancer, Lyon, France)|
Research Projects in Limms
Development of a new microscale culture model to investigate the differentiation and maturation of induced pluripotent stem cells to hepatocytes
Context: Hepatocytes differentiated from induced pluripotent stem (iPS) cells emerged as a promising source for in vitro liver model. iPS cells have two main properties: they may differentiate into all cell types that make up the body (pluripotency) and are able in ad hoc conditions to proliferate indefinitely in culture (self-renewal). Despite encouraging results, hepatocytes derived from iPS cells still present a differentiation pattern of primitiveness (AFP, SOX17) illustrating an incomplete maturation.
Objectives & Methods: The project liver-iPS on-chip aims to develop, from hepatocytes derived from iPS cells, a microfluidic liver tissue culture model, which will provide 3D, dynamics and mass transfer nutrient control in a micro-confined environment mimicking in vivo situations. This microfluidic bioreactor will then be used in epigenetic and hepatotoxicity studies.