Dominique COLLARD, Dr.

limms_DCo.jpg Host Laboratory FUJITA LAB.
Position in LIMMS CNRS director of research (from August 2005 to )
Main Research Topic in LIMMS Advanced-MEMS - Bio-mechanical sensing of enzymatic reaction on DNA bundle by silicon nanotweezers
Keywords Nanotweezers, Molecules, DNA, Enzyme, Biosensing
Contact LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088

E-mail

collard at iis.u-tokyo.ac.jp

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icon_pdf.gifAbstract2015_DCollard.pdf ,  icon_pdf.gifResume D Collard ,

Resume

  Dominique Collard was born in Cambrai, France in 1958. He received the Eng. Degree from ISEN (Institut Supérieur d'Electronique et du Numérique) in 1980, and the PhD degree from the University of Lille in 1984. From 1985 to 1986, he was with TOSHIBA ULSI research Center in Kawasaki, Japan, as visiting scientist. He entered the Centre National de la Recherche Scientifique (CNRS) as senior researcher in 1988, and settled a research group on process and device simulation at ISEN and IEMN (Institut d’Electronique, de Microélectronique et de Nanotechnologie), Lille, France. In 1995-1997, he was Director of the Laboratory for Integrated Micromechatronic Systems (LIMMS), Tokyo, Japan, a joint CNRS laboratory with the Institute of Industrial Science of the University of Tokyo. Within LIMMS he worked on silicon based electrostatic actuator. From 1997, he is with IEMN, as CNRS research director and settled a silicon micro-system group. November 2000-July 2005, he has a Professor position at the University of Tokyo and was Director of the CIRMM/CNRS (Center of International Research on MicroMechatronics). From August 2005, he joint a second time the Laboratory for Integrated Micromechatronic Systems (LIMMS), Tokyo, Japan where he was appointed director in Sept 2007.  In Dec. 2011, he became coordinator of EC/FP7 INCOLAB: EUJO-LIMMS aiming to open LIMMS to European partners and first EC laboratory in Japan. His current scientific interest covers micro and nano systems for applications in biology and nanotechnology.

Main publication List (before LIMMS)
  1. D. COLLARD and K. TANIGUCHI, “IMPACT, a point defect based two-dimensional process simulator: modeling of lateral oxidation enhanced diffusion of dopants in silicon”, IEEE Trans. Electron Devices, Vol. ED-33, pp. 1454-1462, 1986.
  2. V. SENEZ, D. COLLARD, B. BACCUS, M. BRAULT and J. LEBAILLY, "Analysis and application of viscoelastic model for silicon oxidation", J. Appl. Phys., Vol. 76, pp. 3285-3296, 15 sept. 1994.
  3. T. AKIYAMA, D. COLLARD and H. FUJITA "Scratch Drive Actuator with mechanical links for Self-assembling of three-dimensional MEMS", J. Microelectromechanical Syst., Vol. 6, pp. 10-17,1997.
  4. D. GALAYKO, A KAISER, L. BUCHAILLOT, B. LEGRAND, D. COLLARD, C. COMBI, "Design, realisation and test of micro-mechanical resonators in thick-film silicon technology with postprocess electrode-to-resonator gap reduction", Journal of Micromechanics and Microengineering, Vol. 13, pp. 134-140, Janvier 2003.
  5. V. AGACHE, B. LEGRAND, D. COLLARD, L. BUCHAILLOT, H. FUJITA, " Fabrication and characterization of 1.1 GHz blade nano-electromechanical resonator", Applied Physics Letters, vol 86, p. 213114, 2005.

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Research Projects in Limms

1. Continuous real-time monitoring of molecular detection by silicon nanotweezers-integrated microfluidic device (with Fujita lab.)

 

There have been numerous successful attempts to build functional molecular microfluidic devices. However, issues on robustness, standardization and reliability of the detection mechanism are not cleared. Fluorescence imaging damages molecules preventing from real-time long-period monitoring while immunoassays suffers standardization problem at low concentrations and electrical measurements have performance limitation in buffer environment. Molecular biomechanical detection with the integrated Silicon Nano Tweezers (SNT) is a promising approach as already demonstrated in air and in liquid. However, performing all steps of the experiment in liquid requires very stable and reliable microfluidic integration.

This research proposes using a PDMS channel, placed on the edge of a cover slip, with a lateral opening for SNT tips to enter (Fig.1). After capturing necessary molecules, input solution changes (with an integrated syringe pump) for detection. The result is monitored as electrical signals showing the change in resonance frequency (Res. Freq.) and quality factor (Q-factor). With this integrated SNT with microfluidics we have demonstrated continuous, real-time monitoring of fluid handling and microtubule (MT) capturing. These results pave the way to molecular detection outputting electrical signals, an awaited reliable indicator to standardize the detection.

 Figure1_collard.png

Fig.1 Schematic view of the developped system. a) SNT was inserted into the channel via the lateral opening of the syringe pump integrated PDMS device. b) The liquid inside the channel was changed with syringe pump. Changes in the resonance frequency and Q-factor monitor MT capturing. The device can then be used for monitoring molecular attachment on MTs.

 
2. Optimized micro devices for liquid-dielectrophoresis (LDEP) actuation of conductive solutions. (with V. Agache, CEA/LETI, Grenoble, France and Fujita Lab.).

 

Liquid dielectrophoresis (LDEP) technique is known to displace liquids in open environments by applying alternating or DC voltages between electrodes patterned below a single or a stack of dielectric layers (Fig.2). This technique is able to handle pico–nano liter droplets and could be used to carry out biological and chemical protocols. According to the electrode geometry, the dielectric layers constants and thicknesses, this technique may require relatively high actuation voltages (∼200–500 VRMS). In addition, most of experiments carried out in the literature have shown the LDEP technique to be more adapted to insulating or semi insulating liquids (lower than 1 mS m−1).

This research reports optimization of LDEP devices, in terms of miniaturization (generation of droplets arrays with droplets size ranging from 0.5 pL to 0.1 nL), and choice of dielectric materials. We have successfully addressed two challenging constraints in the field of LDEP: firstly deionized water can be actuated with operating voltages below 150 VRMS and secondly solutions with conductivity up to 10 mS m-1 have been partly displaced. These results allow us to foresee manipulation of organic solutions, biological buffers by LDEP at the micro-nanoscale, which will attract strong interest for sample preparation in complex biochemical protocols.

Figure2_collard.png




Fig. 1 (a)–(c) Evolution of a DI water liquid finger while applying a 250 ms signal pulse of 236 VRMS at 100 kHz. The dielectric layers are made of 300 nm SiN and 300 nm SiOC. (d)–(e) The picoliter droplets are created by the break-up of the capillary instability at semi circular profiles positions called bumps.

Figure2b_collard.png

Fig. 2 Graph illustrating the influence of the geometry (electrode width w and inter electrode gap g) onto the threshold actuation voltage Vth.

 
3. Silicon nano tweezers for real time biomechanical assay on dna damage by therapeutic radiation beams (with Center Oscar Lambret, Lille, France and Fujita lab.).

 

Tumor cell killing by γ-ray beams in cancer radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. On the other hand, the mechanical behavior of DNA, e.g., sequence–sensitivity, elastic vs. plastic response, is well understood. However, manipulations are usually performed by AFM or optical tweezers, instruments that can hardly be placed and operate under radiation beams. This new activity aims to the biomechanical characterization of a λ-DNA bundle exposed to a therapeutic radiation beam by Silicon Nanotweezers (Fig. 3). The device endures the harsh environment of radiation beams and still retains molecular-level accuracy. This result paves the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment.

Figure3a_collard.jpg

Fig. 3 (a) CyberKnife machine in Centre Oscar Lambret, Lille, France used for the experiments.

Figureb_collard.jpg

Fig. 3 (b) Experimental set-up. The SNT is under the radiation beam generated by the CyberKnife head. (c) Picture of the SNT showing the grounded connection to the lock-in amplifier (not shown).

4.  Bio-mechanical sensing of enzymatic reaction on DNA bundle by silicon nano tweezers


Abstract :
The objective of this project is to demonstrate, for the first time, the single molecule manipulation and characterization by micromachined silicon based tweezers. DNA is the first targeted molecule. Molecule isolation is performed thanks to microfluidics device (Fig.1). Dedicated tweezers have been developed with appropriate actuation and sensing characteristics (Fig.2). DNA bundle has been successfully trapped by immersed tweezers, as shown by SEM image of a trapped DNA bundle bridging the tweezers tips (Fig. 3).

dCo01.jpg   dCo02.jpg   dCo03.jpg

   icon_pdf.gif Project

Context :

Performing biological test by direct molecular manipulation can provide quick and reliable data as noise due to bulk experiment can be reduced. However, these experiments relying on optical or magnetic tweezers have a low throughput since the molecule preparation is done one at a time. To move towards systematic biological or medical analysis, Micro and Nano Systems (MEMS) are the appropriate tools as they can integrate accurate molecular level engineering tools and can be cheaply produced with highly parallel process. This research focuses on this long term goal.

Objectives & Methods :

The objective of this project is to demonstrate routine molecules manipulation and systematic bio reaction sensing by Silicon Nano Tweezers (SNT), Fig.1. DNA molecules bundle is trapped by the SNT from DNA solution. The biological reaction is sensed by immersing the trapped bundle in enzyme solution and tracking the mechanical response of the SNT.

Results :

DNA bundle is first trapped between the 2 tips of the tweezers, insert of Fig. 1. The bundle handled by the SNT is immersed in the enzymatic solution contained in the reaction cell. The enzymes, Hind III digest the DNA. The mechanical resonance frequency FR and Q factor of the SNT+bundle is extracted in real time as shown in fig. 2. The reduction of FR  and increase of the Q factor give the kinetics of the digestion reaction. This first result proves the feasibility of the molecular based bio-sensing with Silicon Nano Tweezers.

           limms_booklet_fig1V2.jpegFig. 1 Silicon Nanotweezers and trapped DNA bundle.

collard_fig2.jpg

Fig. 2 Tweezers + bundle FR and Q vs. Digestion time. Response without proteins is also shown (witnesses).

References and Publications :

[1] M. Kumemura et al., J. Micromech. Microeng, 21 (054020), 2011.

[2] Collard, Front Biosci, 5 (955), 2013.

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Publications in LIMMS

Main publication List (papers, conferences and patent)

2016

Journals (submitted)
      1. Y. Tauran, C. M. Tarhan, L. Mollet, J., et al., "Elucidating the mechanism of the considerable mechanical stiffening of DNA induced by the couple Zn2+/Calix[4]arene-1,3-O-diphosphorous acid," Chem. Comm., p. submitted, 2016
      2. C. M. Tarhan, R. Yokokawa, L. Jalabert, D. Collard, and H. Fujita, "Pick-and-place assembly of single microtubules by MEMS tweezers," Small, p. submitted, 2016
      3. A. Shkurenko, A. N. Lazar, A. Navaza, D. Collard, M. C. Tarhan, B. Kim, et al., "The Solid-State Structures of Organic Salts Formed by Calix[4]arene Dihydroxyphosphonic Acid with Nucleic Bases Cations: Adeninium, Cytosinium, Guaninium and Uracilium," RSC Advances, p. submitted, 2016
      4. I. Montassier, A. W. Coleman, Y. Tauran, L. Jalabert, D. Collard, B. Kim, et al., "Direct measurement of Magnesium specific modification of the mechanical properties of DNA," Chem. Comm., p. submitted, 2016
      5. M. Danoy, M. Shinohara, A. R. Safitri, D. Collard, V. Senez, and Y. Sakai, "Alteration of pancreatic carcinoma cells adhesion in the liver microvasculature by coculture of hepatocytes, pericytes and endothelial cells in a physiologically-relevant model," Integrative Biology, p. submitted, 2016

 

Journals
      1. M. C. Tarhan, N. Lafitte, Y. Tauran, L. Jalabert, M. Kumemura, G. Perret, et al., "A rapid and practical technique for real-time monitoring of biomolecular interactions using mechanical responses of macromolecules," Scientific Reports, vol. 6, p. 28001, 06/16/online 2016
      2. G. Perret, T. Lacornerie, F. Manca, S. Giordano, M. Kumemura, N. Lafitte, et al., "Real-time mechanical characterization of DNA degradation under therapeutic x-ray and its theoretical modelling," microsyst. nanoeng., p. In Press, 2016
      3. G. Perret, P. Ginet, M. C. Tarhan, A. Baccouche, T. Lacornerie, M. Kumemura, et al., "Nano systems and devices for applications in biology and nanotechnology," Solid-State Electronics, vol. 115, Part B, pp. 66-73, 2016
      4. M. Loeblein, G. Perry, S. H. Tsang, W. Xiao, D. Collard, P. Coquet, et al., "Three-Dimensional Graphene: A Biocompatible and Biodegradable Scaffold with Enhanced Oxygenation," Advanced Healthcare Materials, vol. 5, pp. 1177-1191, 2016
      5. S. L. Karsten, M. Kumemura, L. Jalabert, N. Lafitte, L. C. Kudo, D. Collard, et al., "Direct electrical and mechanical characterization of in situ generated DNA between the tips of silicon nanotweezers (SNT)," Lab on a Chip, vol. 16, pp. 2099-2107, 2016

 

Additional documents related to "Scientific Reports, vol. 6, p. 28001, 06/16/online 2016"
      1. M. Koppe, "Une nanopince pour analyser les caractéristiques mécaniques des macromolécules", CNRS Innovation, http://www.cnrs.fr/lettre-innovation/actus.php?numero=352, vol. 22 Juin 2016, ed. Paris: Editions du CNRS 2016
      2. Real-time mechanical characterization of DNA degradation under therapeutic x-ray and its theoretical modelling," microsyst. nanoeng., p. In Press, 2016

 

2015

Journals
      1. S.L. Karsten, M.C. Tarhan, L.C. Kudo, D. Collard, H. Fujita, Point-of-Care Devices (POCDs) by Means of Advanced MEMS, Talanta, 2015 (accepted). 
Conferences
      1. D. Collard, Nano systems and nano scaled devices for new applications in biology and nanotechnology. (invited plenary talk), EUROSOI-ULIS 2015, 2015 Joint International EUROSOI Workshop and International Conference on Ultimate Integration on Silicon, January 26-28, 2015 - Bologna, Italy.

 

2014

Journals
      1. R. Renaudot, Y. Fouillet, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, et al., "Performances of a broad range of dielectric stacks for Liquid Dielectrophoresis transduction," Microfluidics Nanofluidics, vol. 16, pp. 620-626, 2014.
      2. R. Renaudot, Y. Fouillet, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, et al., "Programmable LDEP technology to fabricate versatile master molds for PDMS continuous-flow microfluidic applications," Microfluid Nanofluid, vol. 16, pp. 701-710, 2014.
      3. N. Lafitte, Y. Haddab, Y. Le Gorrec, H. Guillou, L. Jalabert, D. Collard, et al., "Improvement of silicon nanotweezers sensitivity for mechanical characterization of biomolecules using closed- loop control," IEEE/ASME Trans. Mechatron., vol. Accepted, 2014.
      4. D. Collard, N. Lafitte, H. Guillou, M. Kumemura, L. Jalabert, and H. Fujita, "Silicon Nano Tweezers for molecules and cells manipulation and characterization," in Emerging Tools for Micro and Nano Manipulation, X. Liu and Y. Sun, Eds., ed: Wiley-VCH, 2014, p. (in press).
Conferences
      1. F. Manca, S. Giordano, P.L. Palla, G. Perret, E. Lartigau, D. Collard, H. Fujita, F. Cleri, "Theoretical study of the basic mechanisms of DNA damage by therapeutic radiation beams", E-MRS Spring meeting 2014, Symposium N, May 26-29, Lille (France).
      2. G. Perret, T. Lacornerie, M. Kumemura, H. Guillou, L. Jalabert, E. Lartigau, T. Fujii, F. Cleri, H. Fujita, D. Collard, "Silicon nanotweezers inside liquid for the real time characterization of DNA degradation under radiotherapy treatment", E-MRS Spring meeting 2014, Symposium N, May 26-29, Lille (France).
      3. M. Kumemura, S. L. Karsten, N. Lafitte, H. Guillou, L. Jalabert, H. Fujita, D. Collard, ELECTRICAL DETECTION OF AMPLIFIED DNA USING SILICON NANOTWEEZERS, 7th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT2014), 11-3 (2pages), June 29 - July 2, 2014 / EXCO, Daegu, Korea.
      4. H. Guillou, N. Iwanaka, N. Lafitte, M. Kumemura, K. Shimizu, L. Jalabert, S. Kaneda, T. Fujii, H. Fujita, K. Sakata, S. Konishi, D. Collard, SILICON NANO TWEEZERS WITH FORCE/STIFFNESS/LOSSES MEASUREMENTS OF LOCAL CONTRACTILITY OF MYOCYTES, 7th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT2014), 11-6 (2pages), June 29 - July 2, 2014 / EXCO, Daegu, Korea.
      5. P-T. Chiang, M. Kumemura , H.Fujita, G. Perret, N. Lafitte, L.Jalabert, D. Collard, A. Enomoto, K. Miyagawa, Real-time Measurement of DNA Degradation under Radiation by Silicon Nanotweezers Coupled with Microfluidic Cavity, Technical Meeting on Sensors and Micromachines 2014.

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2013

Journals
      1. R. Renaudot, V. Agache, Y. Fouillet, G. Laffite, L. Jalabert, E. Bisceglia, M. Kumemura, D. Collard and H. Fujita, "A programmable and reconfigurable microfluidic chip", Lab Chip,Lab Chip, 2013,13, 4517-4524.
      2. R. Renaudot, Y. Fouillet, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, V. Agache “Programmable LDEP technology to fabricate versatile master molds for PDMS continuous-flow microfluidic applications”, Microfluidics and Nanofluidics, Sept. 2013.
      3. R. Renaudot, V. Agache, Y. Fouillet, M. Kumemura, L. Jalabert, D. Collard, H. Fujita, Performances of a broad range of dielectric stacks for liquid dielectrophoresis transduction, Microfluidics Nanofluidics, 2013.
      4. M. Usami, T. Ishida, M. Kumemura, L. Jalabert, D. Collard and H. Fujita, "Four-Terminal Electrical Measurement of a DNA Molecular Bundle Captured by Silicon Nanotweezers", IEEJ Transactions on Sensors and Micromachines, Vol. 133 - 3, pp. 98-99 (2013)
      5. R. Renaudot, T. Nguyen, Y. Fouillet, D. Jary, M. Kumemura, L. Jalabert, D. Collard, H. Fujita and V. Agache, "Alternative method for local surface functionalization using liquid dielectrophoresis: An application with polyethyleneimine polymer for bacteria trapping onto a surface", RSC Advances (2013)
      6. R. Renaudot, B. Daunay, M. Kumemura, V. Agache, L. Jalabert, D. Collard and H. Fujita, "Optimized micro devices for liquid-dielectrophoresis (LDEP) actuation of conductive solutions", Sensors and Actuators B: Chemical, Vol. 177 - 0, pp. 620-626 (2013)
      7. J. Malapert, S. Morishita, M. Ataka, H. Fujita, D. Collard and Y. Mita, "Power-Regulated Thermal Actuator Based on UV-Patterned Polyimides for a Ciliary Motion System", IEEJ Transactions on Sensors and Micromachines, Vol. 133 - 3, pp. 77-84 (2013)
      8. D. Collard, S. H. Kim, T. Osaki, M. Kumemura, B. Kim, D. Fourmy, T. Fujii, S. Takeuchi, S. L. Karsten and H. Fujita, "Nano bioresearch approach by microtechnology", Drug discovery today, vol. 18 - 11/12, pp. 552-559 (2013)
      9. D. Collard, "Silicon Nanotweezers for Biomechanical and bioelectrical essays", Frontiers in Bioscience, Invited review, Vol. E5, pp. 955-965 (2013)
Conferences
      1. G. Perret, T. Lacornerie, M. Kumemura, N. Lafitte, H. Guillou, L. Jalabert, E. Lartigau, T. Fuji, F. Cleri, H. Fujita, D. Collard, Real Time Biomechanical Characterization of DNA Damage under Therapeutic Radiation Beams, MRS Boston, Dec. 3rd 2013 (Oral).
      2. M. Kumemura, S. L. Karsten, N. Lafitte, H. Guillou, L. Jalabert, H. Fujita, D. Collard, Isothermal amplification of DNA on tips of silicon nanotweezers and its electrical and mechanical characterizations,  MicroTAS 2013, October 27-31, 2013, Freiburg, Germany.(oral).
      3. R. Renaudot, T. Nguyen, Y. Fouillet, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, V. Agache, Local surface modification at the microscale enabled by liquid dielectrophoresis, MicroTAS 2013, October 27-31, 2013, Freiburg, Germany.(poster)
      4. G.Perret, T.Lacornerie, M.Kumemura, N.Lafitte, H.Guillou, L.Jalabert, E.Lartigau, T.Fujii, F.Cleri, H. Fujita, D.Collard, Real time bio mechanical characterization of DNA damage under therapeutic radiation beams,  MicroTAS 2013, October 27-31, 2013, Freiburg, Germany. (poster)
      5. N. Lafitte, Y. Haddab, Y. Le Gorrec, H. Guillou, M. Kumemura, L. Jalabert, H. Fujita, D. Collard, Closed-loop control of silicon nanotweezers for improvement of sensitivity to mechanical stiffness measurement and bio-sensing on DNA molecules, 2013 IEEE/RSJ International Conference on Intelligent Robots and Systems (Oral).
      6. H. Guillou, N. Lafitte, L. Jalabert, M. Kumemura, T. Fujii, H. Fujita, D. Collard, SILICON NANO TWEEZERS FOR EASY CELL BIOMECHANICAL MEASUREMENTS, NMC 2013 Workshop, May 1-3, 2013, Stanford, CA, USA. (poster)
      7. J. Lee, K. Yagi, M. Kumemura, T. Sato, L. Jalabert, D. Collard, H. Houjou, and H. Fujita, CHARACTERIZATION OF Π-CONJUGATED METALLOPOLYMER’S MECHANICAL STIFFNESS BY USING SILICON NANOTWEEZERS, IEEE Transducers 2013, June, Barcelona (Spain).(Oral)
      8. H. Guillou, N. Lafitte, R. Léal, L. Jalabert, T. Fujii, H. Fujita, Y. T. Matsunaga, D. Collard, SILICON NANOTWEEZERS WITH DOUBLE ACTUATION AND EXTENDED STROKE FOR SINGLE CELL STUDIES, IEEE Transducers 2013, June, Barcelona (Spain). (poster)
      9. R. RenaudotY. Fouillet, VAgache, MKumemura, LJalabert, D. Collard and H. Fujita, FAST CONTINUOUS PARAFFIN WAX CHANNEL-BASED MICROFLUIDIC CHIPS FABRICATION USING LIQUID DIELECTROPHORESIS AND ELECTROWETTING, IEEE Transducers 2013, June, Barcelona (Spain).(poster)
      10. N. Lafitte, Y. Haddab, Y. Le Gorrec, H. Guillou, M. Kumemura, L. Jalabert, D. Collard and H. Fujita, ACTIVE CONTROL OF SILICON NANOTWEEZERS DETECTS ENZYMATIC REACTION AT THE MOLECULAR LEVEL, IEEE Transducers 2013, June, Barcelona (Spain). (poster).

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2012

Journals
      1. M. Usami, T. Ishida, M. Kumemura, L. Jalabert, D. Collard and H. Fujita, "Four-Terminal Electrical Measurement of a DNA Molecular Bundle Captured by MEMS Tweezers", SEISAN KENKYU, Vol. 64 - 3, pp.329-333 (2012)
      2. M. Kumemura, D. Collard, S. Yoshizawa, B. Wee, S. Takeuchi and H. Fujita, "Enzymatic reaction in droplets manipulated with liquid dielectrophoresis", Chemphyschem, Vol. 13 - 14, pp. 3308-12 (2012)
      3. L. Desbois, A. Padirac, S. Kaneda, A. J. Genot, Y. Rondelez, D. Hober, D. Collard and T. Fujii, "A microfluidic device for on-chip agarose microbead generation with ultralow reagent consumption", Biomicrofluidics, Vol. 6 - 4, pp. 044101 (2012)
Conferences
      1. Dominique Collard, Thomas Lacornerie, Momoko Kumemura, Nicolas Lafitte, Herve Guillou, Laurent Jalabert, Eric Lartigau, Teruo Fujii, Fabrizio Cleri, Hiroyuki Fujita, "SILICON NANO TWEEZERS FOR REAL TIME BIOMECHANICAL ASSAY ON DNA DAMAGE BY THERAPEUTIC RADIATION BEAMS" (Oral), MicroTAS, Okinawa, JAPAN, Oct. 28 – Nov. 1, 2012. 
      2. M. C. Tarhan,1 D. Collard, L. Jalabert, M. Kumemura, N. Lafitte, Q. Delouvee, S. L. Karsten and H. Fujita, "CONTINUOUS REAL-TIME MONITORING OF MOLECULAR DETECTION BY SILICON NANOTWEEZERS-INTEGRATED MICROFLUIDIC DEVICE", MicroTAS, Okinawa, JAPAN, Oct. 28 – Nov. 1, 2012.
      3. Y. Tauran, M. Kumemura, N. Lafitte, R. Ueno, L. Jalabert, Y. Takayama, D. Collard, H. Fujita, A. W. Coleman and B-J. Kim, ‘Mechanical effect of calix[n]arene capped silver nanoparticles on DNA measured with silicon nano tweezers’, MicroTAS, Okinawa, JAPAN, Oct. 28 – Nov. 1, 2012.
      4. R. Renaudot, V. Agache, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, Performances of High-K dielectric materials (Al2O3, HfO2, ZrO2) for Liquid Dielectropphoresis (LDEP) microfluidic devices, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2012), 28 Oct-1 Nov, Okinawa, Japan.
      5. R. Renaudot, Y. Fouillet, V. Agache, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, New approach for continuous based-channels microfluidic chips fabrication using EWOD and/or LDEP transductions, 8th International Meeting on Electrowetting, 21-23 June 2012, Athens, Greece. Best poster award.

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2011

Journals
      1. R. Renaudot, V. Agache, B. Daunay, P. Lambert, M. Kumemura, Y. Fouillet, D. Collard and H. Fujita, "Optimization of Liquid DiElectroPhoresis (LDEP) Digital Microfluidic Transduction for Biomedical Applications", Micromachines, Vol. 2 - 2, pp.258-273 (2011)
      2. M. Kumemura, D. Collard, N. Sakaki, C. Yamahata, M. Hosogi, G. Hashiguchi and H. Fujita, "Single-DNA-molecule trapping with silicon nanotweezers using pulsed dielectrophoresis", Journal of Micromechanics and Microengineering, Vol. 21 - 5, pp.054020 (2011)
      3. T. Ishida, F. Cleri, K. Kakushima, M. Mita, T. Sato, M. Miyata, N. Itamura, J. Endo, H. Toshiyoshi, N. Sasaki, D. Collard and H. Fujita, "Exceptional plasticity of silicon nanobridges", Nanotechnology, Vol.22 - 35, pp.355704 (2011)
Conferences
      1. M. C. Tarhan, R. Yokokawa, L. Jalabert, D. Collard, and H. Fujita, "Assembling Microtubule Networks for a Motor Protein-based Nano Transport System", 2011 MRS Spring Meeting and Exhibit, San Francisco, USA, Apr 26, 2011
      2. B. Daunay, P. Lambert, D. Collard, and H. Fujita, "Etude par plans d’expériences de la generation de goutelettes obtenues par diélectrophorèse liquid", Cong. Franç. de Mécanique, Besançon, France, Aug 28-Sept 2, 2011
      3. M. Kumemura, S.L. Karsten, D. Collard, and H. Fujita, "Rolling Circle Amplification (RCA) and Trapping of DNA on a Microchip", The Asia-Pacific Interdisciplinary, Toyohashi University of Technology, Aichi, Japan, Nov 18, 2011
      4. M. C. Tarhan, R. Yokokawa, L. Jalabert, D. Collard and H. Fujita, A MOTOR PROTEIN-BASED ENZYMATIC DETECTION SYSTEM, accepted to MicroTAS 2011.
      5. N. Lafitte, M. Kumemura, L. Jalabert, D. Collard and H. Fujita, REAL-TIME SENSING OF MOLECULE BINDING ON DNA WITH SILICON NANOTWEEZERS, accepted to MicroTAS 2011.
      6. Y-A. Chapuis, L. Jalabert, E. Sarajlic, D. Collard, H. Fujita, Polymer Membrane-based thermo-pneumatic actuation for distributed air-jet planar micromanipulator, Accepted as an oral presentation at IEEE Int. Conference on MEMS 2011, Cancun, Mexico.
      7. M. Kumemura, D. Collard, R. Tourvielle, N. Lafitte, K. Montagne, S. Yoshizawa, D. Fourmy, L. Jalabert, Y. Sakai, S. Takeuchi, T. Fujii, and H. Fujita, INTEGRATED MEMS PLATFORM WITH SILICON NANOTWEEZERS AND OPEN MICROFLUIDIC DEVICE FOR MOLECULAR AND CELLULAR BIOMECHANICAL ASSAYS, Accepted as an oral presentation at IEEE Int. Conference on MEMS 2011, Cancun, Mexico.
      8. B. Daunay, P. Lambert, L. Jalabert, D. Collard and H. Fujita, Optimization of liquid dielectrophoresis (L-DEP) based devices towards conductive biological liquids handling, Accepted in Transducers 2011, Beijin.
      9. N. Lafitte, M. Kumemura, D. Collard, R. Tourvielle, K. Montagne, S. Yoshizawa, D. Fourmy, L. Jalabert, Y. Sakai, S. Takeuchi, T. Fujii and H. Fujita, “Silicon nanotweezers for molecular and cellular biomechanical assays,” The 6th International Conference on Microtechnologies in Medicine and Biology, Lucerne, Switzerland, MMB 2011.

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2010

Journals
      1. M. C. Tarhan, R. Yokokawa, C. Bottier, D. Collard and H. Fujita, "A Nano-Needle/Microtubule Composite Gliding on a Kinesin-Coated Surface for Target Molecule Transport", Lab Chip, Vol. 10 - 1, pp.86-91 (2010)
      2. F. Cleri, T. Ishida, D. Collard and H. Fujita, "Atomistic simulation of plasticity in silicon nanowires", Applied Physics Letters, Vol. 97 - 15, pp.153106-3 (2010)
Conferences
      1. MC. Tarhan, R. Yokokawa, L. Jalabert, D. Collard, H. Fujita, Biomotor-Based Nanotransport System Constructed by Pick-And-Place Assembly of Individual Molecule, The 2010 IEEE/RSJ International Conference on Intelligent RObots and Systems, Taipei International Convention Center, Taipei, Taiwan, October 18-22, 2010.
      2. N. Lafitte, M. Kumemura, D. Collard, S. Yoshizawa, D. Fourmy, L. Jalabert, S. Takeuchi, T. Fujii, H. Fujita, Direct bio-mechanical sensing analysis of enzymatic reactions on DNA by silicon nanotweezers, Technical Meeting on Micromachine and Sensor Systems, IEE Japan, 2010, June 17-18, Tokyo.
      3. N. Lafitte, M. Kumemura, M. Nagai, L. Jalabert, D. Collard and H. Fujita, “An open microfluidic device with acrive valves for accurate trapping of DNA by silicon nanotweezers," Proc. of 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp. 1865-1867, Groningen, The Netherlands, μTAS 2010.
      4. N. Lafitte, M. Kumemura, D. Collard, S. Yoshizawa, D. Fourmy, L. Jalabert, S. Takeuchi, T. Fujii and H. Fujita, “Direct bio-mechanical sensing of enzymatic reaction on DNA by silicon nanotweezers," IEJ Workshop management system, The papers of technical meeting on Micromachine and Sensor System, The Institute of Electrical Engineers of Japan, pp.127-131, Tokyo 2010.
      5. M. Kumemura, D. Collard, S. Yoshizawa, D. Fourmy, N. Lafitte, L.Jalabert, S. Takeuchi, T. Fujii, H. Fujita, DIRECT BIO-MECHANICAL SENSING OF ENZYMATIC REACTION ON DNA BY SILICON NANOTWEEZERS, IEEE Int. Conf. on MEMS 2010, Hong Kong.

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2009 and prior

Journals

  2009

      1. T. Ishida, Y. Nakajima, J. Endo, D. Collard and H. Fujita, "Real-time transmission electron microscope observation of gold nanoclusters diffusing into silicon at room temperature", Nanotechnology, Vol. 20 - 6, pp.065705 (2009)
      2. D. Collard and H. Fujita, "Mechanical characterization and modeling of chain molecules with MEMS tweezers", IEEJ Transactions on Electrical and Electronic Engineering (2009)
      3. C. Bottier, J. Fattaccioli, M. C. Tarhan, R. Yokokawa, F. O. Morin, B. Kim, D. Collard and H. Fujita, "Active transport of oil droplets along oriented microtubules by kinesin molecular motors", Lab Chip, Vol. 9, pp.1694–1700 (2009)
      4. M. Ataka, B. Legrand, L. Buchaillot, D. Collard and H. Fujita, "Design, Fabrication, and Operation of Two-Dimensional Conveyance System With Ciliary Actuator Arrays", IEEE/ASME Trans. on Mechatronics, Vol. 14 - 1, pp.119-125 (2009)
      5. H. Arata, F. Gillot, D. Collard and H. Fujita, "Millisecond analysis of double stranded DNA with fluorescent intercalator by microthermocontrol-device", Talenta, Vol. 79 - 5, pp.963-966 (2009)

  2008

      1. C. Yamahata, D. Collard, T. Takekawa, M. Kumemura, G. Hashiguchi and H. Fujita, "Humidity Dependence of Charge Transport through DNA Revealed by Silicon-Based Nanotweezers Manipulation", Biophys. J., Vol. 94 - 1, pp.63-70 (2008)
      2. C. Yamahata, D. Collard, B. Legrand, T. Takekawa, M. Kumemura, G. Hashiguchi and H. Fujita, "Silicon Nanotweezers With Subnanometer Resolution for the Micromanipulation of Biomolecules", J. of Microelectromechanical Syst., Vol. 17 - 3, pp. 623-631 (2008)
      3. E. Sarajlic, C. Yamahata, M. Cordero, D. Collard and H. Fujita, "HAREM: high aspect ratio etching and metallization for microsystems fabrication", J. Micromech. Microeng., Vol. 18 - 7, pp.075008 (2008)
      4. H. Miura, J. Sone, D. Collard, C. Yamahata and H. Fujita, "FEM Analysis of Mechanical Characteristics of Nano Tweezers with MemsOne", IEEJ Transactions on Sensors and Micromachines, Vol. 128 - 5, pp.252-256 (2008)
      5. D. Collard, S. Takeuchi and H. Fujita, "MEMS technology for nanobio research", Drug Discovery Today, Vol. 13 - 21-22, pp.989-996 (2008)
      6. L. Buchaillot, O. Millet, E. Quévy and D. Collard, "Post-buckling dynamic behavior of self-assembled 3D microstructures", Microsystem Technologies, Vol. 14 - 1, pp.69-78 (2008)

  2007

      1. E. Sarajlic, D. Collard, H. Toshiyoshi and H. Fujita, "Design and modeling of compliant micromechanism for mechanical digital-to-analog conversion of displacement", IEEJ Transactions on Electrical and Electronic Engineering, Vol. 2- 3, pp.357-364 (2007)
      2. M. Kumemura, D. Collard, C. Yamahata, N. Sakaki, G. Hashiguchi and H. Fujita, "Cover Picture: Single DNA Molecule Isolation and Trapping in a Microfluidic Device" (ChemPhysChem 12/2007), ChemPhysChem, Vol. 8 - 12, pp.1733 (2007)
      3. M. Kumemura, D. Collard, C. Yamahata, N. Sakaki, G. Hashiguchi and H. Fujita, "Single DNA Molecule Isolation and Trapping in a Microfluidic Device", ChemPhysChem, Vol. 8 - 12, pp.1875-1880 (2007)
      4. D. Collard, C. Yamahata, B. Legrand, T. Takekawa, M. Kumemura, N. Sakaki, G. Hashiguchi and H. Fujita, "Towards mechanical characterization of biomolecules by MNEMS tools", IEEJ Transactions on Electrical and Electronic Engineering, Vol. 2 - 3, pp. 262-271 (2007)

 

Conferences

  2009

      1. Bottier C.,Fattaccioli J.,Tarhan M.C.,Yokokawa R.,Kim B.,Collard D. and Fujita H., Kinesin-driven oil droplets used as carriers for transportation of solid particles 13th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 09, Jeju, Korea, 2009
      2. Kumemura M.,Yoshizawa S.,Collard D. and Fujita H., Droplet formation and fusion for enzyme activity measurement by liquid dielectrophoresis, 15th Int. Conf. on Solid State Sensors, Actuators and Microsystems, Transducers 09, Denver, USA, 813-816, 2009
      3. Tahran M.C.,Yokokawa R.,Jalabert L.,Bottier C.,Collard D. and Fujita H., Kinesin motion at microtubule crossings of a nano transportation system: precise analysis realized by single molecular handling and assembly, 13th Int. Conf. on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 09, Jeju, Korea, 2009
      4. Tarhan M.C.,Jalabert L.,Yokokawa R.,Bottier C.,Collard D. and Fujita H., Nano monorail for molecular motors: individually manipulated microtubules for kinesin motion, 15th Int. Conf. on Solid State Sensors, Actuators and Microsystems, Transducers 09, Denver, USA, 2164-2167, 2009
      5. Wada K.,Tarhan M.C.,Bottier C.,Collard D.,Fujita H. and Yokokawa R., Fabrication and characterization of multiple nanowires using microtubule structures, 15th Int. Conf. on Solid State Sensors, Actuators and Microsystems, Transducers 09, Denver, USA, 2009
      6. Yamahata C.,Sarajic E.,Collard D.,Jalabert L.,Kumemura M. and Fujita H., Mechanical characterization of biomolecules in liquid using silicon tweezers with subnanonewton resolution, IEEE 22th Int. Conf. on Micro Electro Mechanical Systems, MEMS 09, Sorrento, Italy, 607-610, 2009.

  2008

      1. Bottier, C.; Tarhan, M. C.; Collard, D.; Yokokawa, R.; Fujita, H. Kinesin-based Transportation and Electrofusion of Lipid Vesicles, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS'08, San Diego, USA, Oct. 12-16, 2008.
      2. Sarajlic, E.; Yamahata, C.; Cordero, M.; Collard, D.; Fujita, H. HAREM: High Aspect Ration Etching and Metallization, 21st IEEE Int. Conf. on Microelectromechanical Systems, MEMS'08, Tucson, USA, Jan. 13-17, 2008.
      3. Tarhan, M. C.; Collard, D.; Bottier, C.; Yokokawa, R.; Hosogi, M.; Hashiguchi, G.; Fujita, H. Isolation and Manipulation of Single Microtubule by Silicon Microtweezers, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS'08, San Diego, USA, Oct. 12-16, 2008.
      4. Wee, B.; Kumemura, M.; Collard, D.; Fujita, H. Isolation of Single DNA Molecule in Picolitre-sized Droplet Formed by Liquid Dielectrophoresis, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS'08, San Diego, USA, Oct. 12-16, 2008.
      5. Wee, B.; Kumemura, M.; Collard, D.; Fujita, H. Integration of Closed Microfluidics and Open Droplet Actuation on a Single Lab-on-a-chip Device, Intelligence and Precision Equipment IIP 2008, Tokyo, Japan, 2008.
      6. Wee, B.; Kumemura, M.; Collard, D.; Fujita, H. Integration of Microfluidics and Droplet Manipulation of DNA Solution on a Single Lab-on-a-chip Device, 4th Asia-Pacific Conference on Transducers and Micro-Nano Technology, APCOT'08, Tainan, Taiwan, Jun. 22-25, 2008.
      7. Wee, B.; Kumemura, M.; Collard, D.; Fujita, H. Droplet Extraction from PDMS Surface using Liquid Dielectrophoresis, International Symposium on Surface Science and Nanotechnology, 2008.
      8. Yamahata, C.; Collard, D.; Domenget, A.; Hosogi, M.; Kumemura, M.; Hashiguchi, G.; Fujita, H. Silicon Nanotweezers: a New Biophysical Tool for Molecular Experimentation, 21st IEEE Int. Conf. on Microelectromechanical Systems, MEMS'08, Tucson, USA, Jan. 13-17, 2008.
      9. Yokokawa, R.; Tarhan, M. C.; Bottier, C.; Collard, D.; Fujita, H.; Kasahara, M. DNA Molecule Manipulation by Motor Proteins and Enzymatic Reaction at the Single Molecule Level, 25th Sensor Symposium on Sensors, Micromachines, and Applied Systems, Okinawa, Japan, Oct.22-24, 2008.

  2007

      1. F. Gillot, H.F. Harata, F.O. Morin, D.Collard, H. Fujita, "Micro heater with Pt Cr thin film thermo-couple for high speed biological measurements", 14 Int. Conf. on Solid State Sensors, Actuators and Microsystems, Transducers'07, 2007, Lyon, France, June 10-14
      2. JB. Bureau, D.Collard, L.Buchaillot, "Compact multi layer piezoresistive gauge for in plane stain measurement in liquidsApplication ", 14 Int. Conf. on Solid State Sensors, Actuators and Microsystems, Transducers'07, 2007, Lyon, France, June 10-14
      3. M. Kumemura, D. Collard, C. Yamahata, N. Sakaki, G. Hashiguchi, H. Fujita, "Isolation of DNA molecule in microchannel and a single molecule trapping between microelectrodes", 11th Int Conf. on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 07, 2007, Paris, France.
      4. N. SakakiM. Kumemura, D. Collard, G. Hashiguchi, H. Fujita, "Trapping of single DNA molecule by MEMS tweezers with pulsed dielectrophoresis", 11th Int Conf. on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 07, 2007, Paris, France.
      5. E.Sarajlic, D. Collard, H. Toshiyoshi, H. Fujita, "12-bit micromechanical digital to analog converter of displacement: Design, Fabrication and Characterization", 20th IEEE Int Conf. on Microelectromechanical Systems MEMS 07, 2007, Kobe, Japan, Jan. 21-25.
      6. C. Yamahata, T. Takekawa, M. Kumemura, G. Hashiguchi, D. Collard, H. Fujita, "Electrical and mechanical charactersitics of DNA bundles revealed by Silicon nanotweezers", 14 Int. Conf. on Solid State Sensors, Actuators and Microsystems, Transducers'07, 2007, Vol. 1, Lyon, France, June 10-14

  2006

    1. C. Yamahata, T. Takekawa, K. Ayano, M. Hosogi, M. Kumemura, D. Collard, G. Hashiguchi, H. Fujita,"Silicon nanotweezers with adjustable gap for the manipulation and characterization of DNA molecules", Proceedings of 2006 International Conference on Microtechnoloies in Medicine and Biology, p.123-124,2006 icon_pdf.gif abstract
    2. M. Kumemura, K. Tamura, G. Hashiguchi, D. Collard and H. Fujita, "Nano-gap fabrication by focused ion beam for DNA trapping", Proceedings of 2006 International Conference on Microtechnoloies in Medicine and Biology, p.271-272,2006 icon_pdf.gif abstract
    3. C. Yamahata, A. Tixier-Mita, B. Lepioufle, H. Suzuki, E. Sarajlic, M. Kumemura, S. Takeuchi, D. Collard and H. Fujita, "Batch fabricated micro and nanopores in silicon for particle translocation detection", accepted at Microtas 2006, Nov. 5-9, Tokyo, Japan, 2006
    4. M. Kumemura, C. Yamahata, N. Sakaki, G. Hashiguchi, D. Collard and H. Fujita, "Measurement and modeling of DNA motion in microchannel including frequency dependent electrophoresis and electro-osmotic flow", accepted at Microtas 2006, Nov. 5-9, Tokyo, Japan, 2006.

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