Satoko YOSHIZAWA, Dr.

 limms_Satoko.jpg Host Laboratory TAKEUCHI LAB.
Position in LIMMS Former CNRS Researcher
Main Research Topic in LIMMS Bio-MEMS - Development of microdevice for high-throughput functional assay of membrane proteins
Contact LIMMS/CNRS-IIS (UMI 2820)
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088
E-mail -
Download icon_pdf.gifAbstract2010_SYoshizawa.pdf

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Research Projects in Limms

1- Development of microdevice for high-throughput functional assay of membrane proteins
Context :
Membrane proteins play important roles in cellular functions. Most existing assay systems of membrane proteins utilize cells harboring the target membrane protein or the membrane isolated from those cells.  An abundant expression and display to the cell membrane is required which is often difficult. A general approach is awaited that is applicable to a wide variety of membrane proteins.
Objectives :
The objective is to develop a microdevice suitable for high-throughput screening of membrane protein function.  Such devices may be used for a fundamental research to understand cellular response to various stimulations as well as for applications such as drug screening and highly-sensitive biosensors.
Methods :
A microfluidic device that generates monodisperse unilamellar phospholipid vesicles was constructed.  Using this device, in vitro translational system can be encapsulated in the vesicles and GFP (green fluorescent protein) can be synthesized inside [1],[2].  We also developed a method to synthesize proteins within PDMS (poly(dimethylsiloxane)) microchambers (c.a. 15µm x 15µm x 15µm) by coating the surface of the chambers with lipids [3]. These microchambers will be further sealed with lipid bilayer.  We used these systems to synthesize membrane proteins and construct microdevices suitable for their functional assays.
References :
[1] S. Ota, S. Yoshizawa and S. Takeuchi, Angew. Chem. Int. Ed. 48, p.6533-6537, 2009.
[2] S. Ota, S. Yoshizawa and S. Takeuchi, Proc. MicroTAS 2009, p1300-1302, 2009.
[3] T. Osaki, S. Yoshizawa and S. Takeuchi, Proc. MicroTAS 2009, p1350-1352, 2009.

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Publications in LIMMS

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