Yusuf ARIK

 Yusuf Arik.jpg Host Laboratory FUJITA LAB.
Position in LIMMS EUJO-LIMMS Researcher (MESA+)
Main Research Topic in LIMMS

NANOTECH - Fluidic TEM device for visualization of biological structures


 TEM, Liquid cell, Nanoparticles, Nanotechnology


Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
Phone:+81 (0)3 5452 6036 / Fax:+81 (0)3 5452 6088


MESA+ Institute for Nanotechnology, University of Twente
P.O. Box 217, 7500 AE Enschede, The Netherlands
Phone: + 31 53 489 2715

E-mail  yb-arik at iis.u-tokyo.ac.jp
Download icon_pdf.gifAbstract2015_YArik.pdf , Abstract2016


Short resume :
2012-now Actual position
2008-2012 Here
2004-2008 Here
2001-2003 Here

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Research Projects in Limms

1- Fluidic TEM device for visualization of biological structures
Context :
Electron microscopy provides high resolution that is not achievable by other techniques. However, it must be carried out in a high vacuum environment and thus it does not allow real time imaging of processes in liquid media. Moreover, specimens undergo difficult and destructive methods. Consequently, electron microscopy is limited to frozen or dry specimens without any guarantee that the sample is captured at the right moment. Furthermore, imaging of fixed specimens does not capture the dynamics of a process, and sample preparation may affect the structure of the object to be imaged [1]. Recent advances in microfabrication and thin film technology allowed fabrication of liquid cells where liquid can be sealed within the device while the microscope is maintained at high vacuum. The observation window is made of materials such as thin silicon nitride films to minimize electron scattering and increasing resolution while providing robust windows for liquid cells [2].
Objectives & Methods :
The objective of this project is visualization of nano-vesicles and bilayer membranes inside the liquid under TEM. For this purpose, a liquid TEM cell was designed and fabricated at the MESA+ Institute in the Netherlands (Fig-1). This design aims at both achieving high resolution through using a very thin membrane and liquid layer and improving device handling by removing the need for assembly. As soon as channel filling is observed under the optical microscope, inlet holes are sealed with epoxy.
Results :
Presence of liquid was successfully observed inside the liquid TEM cell under vacuum conditions with moving gold nanoparticles in water (Fig-2). As a proof of concept, this shows potential for observing biological structures in liquid under TEM. Methods for successive sealing and improved resolution are currently being investigated.


Fig. 1 Cross-sectional schematics of Liquid TEM cell.


Fig. 2 Filled vs. empty nano-channels.

References :
[1] J.M. Grogan et al. Journal of the Indian Institute of Science. 92:295-308, 2012.
[2] N. de Jonge et al. Nature nanotechnology. 6:695-704, 2011.

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Main publication List (papers, conferences and patent)









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