Adsorption and combing of DNA on HOPG surfaces

Researcher(s)

  • Contact Researcher: Franck ROSE, Dr. - Pascal MARTIN, Dr.
  • Hosted LIMMS Japanese Laboratory: Kawakatsu Lab --- Micro Components and Systems

Project Overview

  • Keywords
    • AFM
    • DNA
    • combing
    • HOPG nanosheets
    • HOPG/Si heterostructures

  • Context :
  • Objectives :
  • Methods :
Controlled and reproducible combing of lambda;-phage DNA molecules can be realized in predetermined orientations on highly oriented pyrolitic graphite (HOPG) surfaces. Observations by atomic force microscopy (AFM) show that DNA adsorption onto HOPG surfaces leads to different hierarchical organizations such as balls, networks, films, and fractal structures. HOPG nanosheets (3.5 to 100 nm thick) were created by simply rubbing a HOPG crystal onto a silicon oxide surface, and then patterned with a focused ion beam (FIB) to fabricate HOPG/Si heterostructures (arrays of silicon micropillars and microtracks decorated on their top surface with HOPG nanosheets). The surface reactivity toward DNA of HOPG nanosheets is found to be the same as of HOPG bulk crystals. Finally, combing is used to attach and suspend bundles of approximately 20-50 DNA molecules between HOPG/Si heterostructures.

Report(s) and Publication(s)

  • References :
F.Rose, P.Martin, H.Fujita, and H.Kawakatsu, Adsorption and Combing of DNA on HOPG Surfaces of Bulk Crystals and Nanosheets: Application to the Bridging of DNA between HOPG/Si Hetero-structures, Nanotechnology 17, 3325 (2006).
P. Martin, F. Rose, F. Morin, H. Fujita and H. Kawakatsu, "FIB - created HOPG/SiO2 heterostructures for adsorbed and suspended DNA", IEEE Technical Digest of International Conference on Microtechnologies in Medicine and Biology (MMB2006), p.173, 2006.
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