Cell culture/ Tissue Engineering in 3D microbioreactors
- Contact Researcher: Christophe PROVIN, Dr.
- Hosted LIMMS Japanese Laboratory: FUJII Lab. (Microbiochemical Systems)
- Research Partner(s) : SAKAI Lab. (Biology and Integrative Medicine)
- liver cell
- optimized design
- Context :
This project is centered on the use of microfabrication techniques for biological applications. Depending on the type of cells which one wishes to cultivate, the needs for those to ensure their development are different. In particular, some (as the cells of the liver) require a significant and regular contribution in nutrients and oxygen in order to keep the specific functionalities they exhibit in vivo.
- Objectives :
For liver, this contribution is being ensured in vivo by a very developed vascular network, so it can be possible to imitate that in vitro by the use of a dense network of microchannels. Moreover, this type of cells is often three dimensionally organized, which implies a structuration of the surface on which culture is done.
- Methods :
Different types of optimized structures will be tested. Those are conceived in collaboration with Shirakashi Laboratory (IIS, Univ. Of Tokyo). The microfabrication will be done with two techniques: softlithography for generation of an extruded two-dimensional PDMS (polydimethylsiloxane) reactor for ex vivo applications such as drug testing, and direct manufacture of the three-dimensional thick and complex biodegradable structures built by microstereolithography for in vivo applications such as implantable artificial liver.
The culture of HepG2 cells (human cancerous liver cells) will make it possible to determine if a high density of viable cells can be reached with those optimized microbioreactors.
Thus, the realization of an effective microbioreactor for liver cells requires the optimization of several parameters: the structure of the microbioreactor must allow a homogeneous distribution of the nutriments and oxygen together with offering surface/volume sufficient to obtain a high density of cells, and the material must be compatible with the chosen process of microfabrication plus the cell culture (biocompatibility).
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