PDMS Microfluidic Device for Sensing Dynamic Response of Cells and Tissues

Researcher(s)

  • Contact Researcher:
    Nazare PEREIRA RODRIGUES, Dr.

  • Hosted LIMMS Japanese Laboratory:
    FUJII Lab. --- Microbiochemical Systems

Project Overview

  • Keywords:
    • Development of electrochemical sensors adapted to biological systems: microfabrication and surface modification
    • Integration of multiple electrochemical sensors in microfluidic devices
    • Development of analytical tools: optico-electrochemical sensor systems

 

  • Context:
Recently, the micro total analysis system (micro-TAS) has attracted widespread attention due to its excellent potential for offering highly efficient and on-line analysis of biological molecules or metabolisms by integrating the microsystem in a microfluidic device.
  • Objectives:
The aim of this study is to develop a novel cell-based microfluidic device that will allow a complete, precise and real-time monitoring of the composition and modulation of cell medium (oxygen, glucose, sodium and potassium ions). Thus, the device is realised by integrating an array of specific sensors in the inlet and outlet of a polydimethylsiloxane (PDMS) microfluidic device . The array of electrochemical microsensors is fabricated by thin film technology and is composed of gold working microelectrodes, a gold counter electrode and an Ag/AgCl reference electrode (Figure 1b).
  • Methods:
First experiments focus on the monitoring of local concentration of oxygen in a PDMS microchannel . The array of gold microelectrodes is covered by a layer of Nafion, which is a gas permeable membrane that enhances the lifetime of the electrodes. Oxygen calibration showed good performances of the amperometric sensor (linearity of response, lifetime and sensitivity) in a physiological buffer medium . Results obtained for O2 monitoring are therefore very promising and further experiments concerning kinetics of oxygen and simultaneous detection of oxygen and glucose will be done with cell cultures (hepatocytes) that will be introduced in the PDMS chamber directly connected to the microchannel containing the electrodes.

 

Photo_device_nazare1.jpg Photo_device_nazare2.jpg
Microfluidic device with integrated sensors Array of electrochemical sensors
in a microfluidic channel

Report(s) and Publications

  • PDF Project Report :

icon_pdf.gif Project LIMMS Fujii Lab nazare

  • References :
  1. N. Pereira Rodrigues et al., MMB 2006, Okinawa, Japan (2006)
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